Western Blot (WB)
(Western Blot analysis in Hela cell line lysates (35ug/lane) using MBS646505. This demonstrates that MBS646505 detected the AKR1C3 protein (arrow).)
Rabbit anti-Human AKR1C3 Polyclonal Antibody | anti-AKR1C3 antibody
AKR1C3, ID (AKR1C3, DDH1, HSD17B5, KIAA0119, PGFS, Aldo-keto reductase family 1 member C3, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-HSD type II, brain, 3-alpha-hydroxysteroid dehydrogenase type 2, Chlordecone reductase homolog HAKRb, Dihydrodi
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Western Blot (WB)
(Western Blot analysis in Hela cell line lysates (35ug/lane) using MBS646505. This demonstrates that MBS646505 detected the AKR1C3 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in Hela cell line lysates (35ug/lane) using MBS646505. This demonstrates that MBS646505 detected the AKR1C3 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS646505 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646505 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS646505 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646505 for immunohistochemistry.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
Function: Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.
Catalytic activity: Trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH.A 3-alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H.(5Z,13E)-(15S)-9-alpha,11-alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH.Testosterone + NAD+ = androstenedione + NADH.Testosterone + NADP+ = androst-4-ene-3,17-dione + NADPH.Indan-1-ol + NAD(P)+ = indanone + NAD(P)H.
Enzyme regulation: Strongly inhibited by nonsteroidal anti-inflammatory drugs (NSAID) including flufenamic acid and indomethacin. Also inhibited by the flavinoid, rutin, and by selective serotonin inhibitors (SSRIs).
Subcellular location: Cytoplasm.
Tissue specificity: Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate. Ref.2 Ref.4 Ref.5 Ref.6 Ref.8 Ref.14
Sequence similarities: Belongs to the aldo/keto reductase family.
Biophysicochemical propertiesKinetic parameters:KM=142.1 µM for progesterone Ref.6KM=2.37 µM for 5-alpha-dihydrotestosteroneKM=1.0 µM for androstanediolVmax=20.1 nmol/min/mg enzyme with progesterone as substrateVmax=1.8 nmol/min/mg enzyme with 5-alpha-dihydrotestosterone as substrateVmax=4.4 nmol/min/mg enzyme with androstanediol as substrate
Sequence caution: The sequence BAA04619.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on AKR1C3
Similar Products
Product Notes
The AKR1C3 akr1c3 (Catalog #AAA646505) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKR1C3, ID (AKR1C3, DDH1, HSD17B5, KIAA0119, PGFS, Aldo-keto reductase family 1 member C3, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-HSD type II, brain, 3-alpha-hydroxysteroid dehydrogenase type 2, Chlordecone reductase homolog HAKRb, Dihydrodi reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AKR1C3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the AKR1C3 akr1c3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKR1C3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
