Western Blot (WB)
(Western Blot analysis of GLYAT expression in transfected 293T cell line by GLYAT polyclonal antibody. Lane 1: GLYAT transfected lysate (17.93kD). Lane 2: Non-transfected lysate)
Mouse anti-Human GLYAT Polyclonal Antibody | anti-GLYAT antibody
GLYAT (ACGNAT, CAT, GAT, Glycine N-acyltransferase, Acyl-CoA:glycine N-acyltransferase, Aralkyl acyl-CoA N-acyltransferase, Aralkyl acyl-CoA:amino acid N-acyltransferase, Benzoyl-coenzyme A:glycine N-acyltransferase, Glycine N-benzoyltransferase, HRP-1(CL
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot analysis of GLYAT expression in transfected 293T cell line by GLYAT polyclonal antibody. Lane 1: GLYAT transfected lysate (17.93kD). Lane 2: Non-transfected lysate)
Western Blot (WB)
(Western Blot analysis of GLYAT expression in transfected 293T cell line by GLYAT polyclonal antibody. Lane 1: GLYAT transfected lysate (17.93kD). Lane 2: Non-transfected lysate)
NCBI and Uniprot Product Information
NCBI Description
The glycine-N-acyltransferase protein conjugates glycine with acyl-CoA substrates in the mitochondria. The protein is thought to be important in the detoxification of endogenous and xenobiotic acyl-CoA's. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate numerous substrates to form a variety of N-acylglycines, with a preference for benzoyl-CoA over phenylacetyl-CoA as acyl donors. Thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid. Ref.6 Ref.7
Catalytic activity: Acyl-CoA + glycine = CoA + N-acylglycine. Ref.6 Ref.7Benzoyl-CoA + glycine = CoA + hippurate. Ref.6 Ref.7
Subcellular location: Mitochondrion Ref.7.
Tissue specificity: Predominantly expressed in liver (at protein level) and kidney. Down-regulated in hepatocellular carcinoma and other liver cancers. Ref.7
Sequence similarities: Belongs to the glycine N-acyltransferase family.
Biophysicochemical propertiesKinetic parameters:KM=57.9 mM for benzoyl-CoA Ref.6KM=83.7 mM for salicyl-CoAKM=124 mM for isovaleryl-CoAKM=198 mM for octanoyl-CoAVmax=17.1 µmol/min/mg enzyme with benzoyl-CoA as substrateVmax=10.1 µmol/min/mg enzyme with salicyl-CoA as substrateVmax=7.64 µmol/min/mg enzyme with isovaleryl-CoA as substrateVmax=3.3 µmol/min/mg enzyme with octanoyl-CoA as substrate
Sequence caution: The sequence AAB81453.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on GLYAT
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Product Notes
The GLYAT glyat (Catalog #AAA646299) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GLYAT (ACGNAT, CAT, GAT, Glycine N-acyltransferase, Acyl-CoA:glycine N-acyltransferase, Aralkyl acyl-CoA N-acyltransferase, Aralkyl acyl-CoA:amino acid N-acyltransferase, Benzoyl-coenzyme A:glycine N-acyltransferase, Glycine N-benzoyltransferase, HRP-1(CL reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GLYAT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the GLYAT glyat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MMLPLQGAQM LQMLEKTLRK SLPASLKVYG TVFHINHGNP FNLKAVVDKW PDFNTVVVCP QEQDMTDDLD HYTNTYQIYS KDPQNCQEFL GSPELINWKQ HLQIQSSQPS LNEAIQNLAA IKSFKVKQTQ RILYMAAETA KELTPFLLKS KILSPSGGKP KAM. It is sometimes possible for the material contained within the vial of "GLYAT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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