Rabbit anti-Human AMPK alpha2 Polyclonal Antibody | anti-AMPK2 antibody

AMPK alpha2, CT (5'-AMP-activated Protein Kinase, Catalytic alpha-2, AMPK Subunit alpha-2, PRKAA2, AMPK, AMPK2) (APC)

Cat. Num. AAA6462737

• Gene Names: PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: AMPK alpha2; Polyclonal Antibody; CT (5'-AMP-activated Protein Kinase; Catalytic alpha-2; AMPK Subunit alpha-2; PRKAA2; AMPK; AMPK2) (APC); anti-AMPK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AMPK alpha2.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-AMPK2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:10; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 453-483 in the C-terminal region of human AMPK alpha2, Swiss/UniProt Accession: P54646, Q09137 rat, Q8BRK8 mouse. Species sequence homology: mouse, rat
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PRKAA2 (arrow) usingMBS624850. 293 cell lysates (2ug/lane) either Lane 1: nontransfected Lane 2: transiently transfected with the PRKAA2 gene (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted withMBS624850, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS624850 for immunohistochemistry.)

Product Categories/Family for anti-AMPK2 antibody

Antibodies; Protein Kinases

References

1. Wyatt, C.N., J. Biol. Chem. 282(11), 8092-8098 (2007). 2. Cheung, S.T., Neoplasia 8(9), 696-701 (2006). 3. Lee-Young, R.S., Am. J. Physiol. Endocrinol. Metab. 291(3), E566-E573 (2006). 4. Gregory, S.G., Nature 441(7091), 315-321 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 46877068
• NCBI GeneID: 5563
• NCBI Accession #: NP_006243
• NCBI GenBank Nucleotide #: NM_006252
• UniProt Accession #: P54646; Q9H1E8; Q9UD43; AAB32732
• Molecular Weight: 62,320 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-2
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, alpha 2 catalytic subunit
• NCBI Official Symbol: PRKAA2
• NCBI Official Synonym Symbols: AMPK; AMPK2; PRKAA; AMPKa2
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; ACACA kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMPK alpha 2
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-2
• UniProt Gene Name: PRKAA2
• UniProt Synonym Gene Names: AMPK; AMPK2; AMPK subunit alpha-2; ACACA kinase; HMGCR kinase
• UniProt Entry Name: AAPK2_HUMAN

Uniprot Description

AMPKA2: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of metabolic enzymes and transcription regulators. Regulates fatty acid synthesis by phosphorylating acetyl-CoA carboxylase. Regulates cholesterol synthesis by phosphorylating and inactivating hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Salicylate/aspirin directly activates kinase activity. Studies in the mouse suggest that AMPK2 may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.27; EC 2.7.11.1; Kinase, protein; Autophagy; Protein kinase, CAMK; EC 2.7.11.31; CAMK group; CAMKL family; AMPK subfamily

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: nucleoplasm; cytosol

Molecular Function: AMP-activated protein kinase activity; protein serine/threonine kinase activity; protein binding; metal ion binding; [acetyl-CoA carboxylase] kinase activity; protein serine/threonine/tyrosine kinase activity; chromatin binding; histone serine kinase activity; ATP binding; protein kinase activity; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity

Biological Process: lipid biosynthetic process; rhythmic process; cellular lipid metabolic process; carnitine shuttle; glucose homeostasis; signal transduction; protein amino acid phosphorylation; cellular response to glucose starvation; cellular response to nutrient levels; regulation of fatty acid biosynthetic process; regulation of transcription, DNA-dependent; response to stress; cell cycle arrest; positive regulation of autophagy; fatty acid biosynthetic process; mitochondrion organization and biogenesis; negative regulation of TOR signaling pathway; Wnt receptor signaling pathway; transcription, DNA-dependent; organelle organization and biogenesis; regulation of circadian rhythm; cholesterol biosynthetic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; autophagy; negative regulation of apoptosis