Rabbit anti-Human, Mouse AKT2 Polyclonal Antibody | anti-AKT2 antibody

AKT2 (RAC-beta Serine/Threonine-protein Kinase, RAC-PK-beta, Protein Kinase Akt-2, Protein Kinase B, beta, PKB beta) (PE)

Cat. Num. AAA6462148

• Gene Names: AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: AKT2; Polyclonal Antibody; AKT2 (RAC-beta Serine/Threonine-protein Kinase; RAC-PK-beta; Protein Kinase Akt-2; Protein Kinase B; beta; PKB beta) (PE); anti-AKT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AKT2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-AKT2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Human AKT2 recombinant protein.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AKT2 Antibody in NCI-H460 cell line lysates (35ug/lane). AKT2 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of AKT2 Antibody in mouse brain tissue lysates (35ug/lane). AKT2 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(AKT2 Antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of AKT2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-AKT2 antibody

Antibodies; Protein Kinases

References

1. Vojtek, A.B., et al., Mol. Cell. Biol. 23(13):4417-4427 (2003). 2. Yuan, Z.Q., et al., J. Biol. Chem. 278(26):23432-23440 (2003). 3. Jones, D.T., et al., Blood 101(8):3174-3180 (2003). 4. Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003). 5. Miyakawa, M., et al., Endocr. J. 50(1):77-83 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4502023
• NCBI GeneID: 208
• NCBI Accession #: NP_001617.1
• NCBI GenBank Nucleotide #: NM_001626.4
• UniProt Accession #: P31751; Q0VAN1; Q68GC0; B2RBD8
• Molecular Weight: 55,769 Da
• NCBI Official Full Name: RAC-beta serine/threonine-protein kinase isoform 1
• NCBI Official Synonym Full Names: v-akt murine thymoma viral oncogene homolog 2
• NCBI Official Symbol: AKT2
• NCBI Official Synonym Symbols: PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
• NCBI Protein Information: RAC-beta serine/threonine-protein kinase; PKB beta; RAC-PK-beta; protein kinase Akt-2; protein kinase B beta; rac protein kinase beta; murine thymoma viral (v-akt) homolog-2
• UniProt Protein Name: RAC-beta serine/threonine-protein kinase
• Protein Family: Potassium channel
• UniProt Gene Name: AKT2
• UniProt Synonym Gene Names: PKB beta
• UniProt Entry Name: AKT2_HUMAN

NCBI Description

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

Akt2: an AGC kinase. Plays critical roles in glucose metabolism and the development or maintenance of proper adipose tissue and islet mass for which other Akt/PKB isoforms are unable to fully compensate. Amplified and overexpressed in human ovarian carcinoma cell lines and amplified in some primary ovarian and pancreatic tumors. Antisense blocks invasiveness in xenografts. Expressed in several insulin-responsive tissues, and one case of Type II diabetes has been associated with a likely LOF point mutation. Mouse mutants have defects in insulin response.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Oncoprotein; AGC group; AKT family

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: nucleoplasm; plasma membrane; cell cortex; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; kinase activity; ATP binding

Biological Process: fat cell differentiation; glycogen biosynthetic process; apoptosis; positive regulation of glycogen biosynthetic process; carbohydrate transport; myelin maintenance in the peripheral nervous system; intracellular protein transport across a membrane; positive regulation of vesicle fusion; protein modification process; glucose metabolic process; signal transduction; regulation of cell migration; protein amino acid phosphorylation; positive regulation of fatty acid beta-oxidation; regulation of translation; positive regulation of glucose import; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation

Disease: Hypoinsulinemic Hypoglycemia With Hemihypertrophy; Diabetes Mellitus, Noninsulin-dependent