Rabbit ACO2 Polyclonal Antibody | anti-ACO2 antibody

ACO2, ID (Aconitate Hydratase, Mitochondrial, Citrate Hydro-lyase, Aconitase) (FITC)

Cat. Num. AAA6461106

• Gene Names: ACO2; ICRD; ACONM
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: ACO2; Polyclonal Antibody; ID (Aconitate Hydratase; Mitochondrial; Citrate Hydro-lyase; Aconitase) (FITC); anti-ACO2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACO2. Species Crossreactivity: mouse, rat.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ACO2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the central region of human Aconitase (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ACO2 Pab in mouse heart(left) and 293 (right) tissue lysates (35ug/lane). ACO2(arrow) was detected using the purified Pab.)

SDS-Page

(Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (Center). Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ?. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.)

Western Blot (WB)

(ACO2 western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Aconitase antibody detected the Aconitase protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human Heart tissue reacted with ACO2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ACO2 antibody

Antibodies; Interferon

References

1. Juang, H.H., Mol. Genet. Metab. 81(3):244-252 (2004). 2. Bota, D.A., et al., Nat. Cell Biol. 4(9):674-680 (2002). 3. Gruer, M.J., et al., Trends Biochem. Sci. 22(1):3-6 (1997). 4. Klausner, R.D., et al., Mol. Biol. Cell 4(1):1-5 (1993). 5. Geurts van Kessel, A.H., et al., Cytogenet. Cell Genet. 28(3):169-172 (1980).

NCBI and Uniprot Product Information

• NCBI GI #: 4501867
• NCBI GeneID: 50
• NCBI Accession #: NP_001089
• NCBI GenBank Nucleotide #: NM_001098
• UniProt Accession #: Q99798; O75809; Q5JZ41; Q6FHX0; Q8TAQ6
• Molecular Weight: 85,425 Da
• NCBI Official Full Name: aconitate hydratase, mitochondrial
• NCBI Official Synonym Full Names: aconitase 2, mitochondrial
• NCBI Official Symbol: ACO2
• NCBI Official Synonym Symbols: ICRD; ACONM
• NCBI Protein Information: aconitate hydratase, mitochondrial; citrate hydro-lyase
• UniProt Protein Name: Aconitate hydratase, mitochondrial
• Protein Family: Aconitate hydratase
• UniProt Gene Name: ACO2
• UniProt Synonym Gene Names: Aconitase
• UniProt Entry Name: ACON_HUMAN

Uniprot Description

ACO2: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. Monomer. Belongs to the aconitase/IPM isomerase family.

Protein type: Lyase; EC 4.2.1.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: mitochondrion; mitochondrial matrix; nucleus

Molecular Function: iron ion binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; 3 iron, 4 sulfur cluster binding

Biological Process: isocitrate metabolic process; cellular metabolic process; generation of precursor metabolites and energy; tricarboxylic acid cycle; citrate metabolic process

Disease: Infantile Cerebellar-retinal Degeneration; Optic Atrophy 8