Rabbit anti-Human, Mouse ACADM Polyclonal Antibody | anti-ACADM antibody

ACADM, ID (Medium-chain Specific Acyl-CoA Dehydrogenase, Mitochondrial, MCAD) (PE)

Cat. Num. AAA6460936

• Gene Names: ACADM; MCAD; ACAD1; MCADH
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ACADM; Polyclonal Antibody; ID (Medium-chain Specific Acyl-CoA Dehydrogenase; Mitochondrial; MCAD) (PE); anti-ACADM antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACADM. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ACADM antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ACADM (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ACADM Antibody (Center) in mouse heart tissue lysates (35ug/lane). ACADM (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ACADM Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of widr cells using ACADM Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ACADM Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-ACADM antibody

Antibodies; Coenzyme A

References

1. Ferreira, A.C., et.al., Genet. Mol. Res. 8(2), 487-493 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4557231
• NCBI GeneID: 34
• NCBI Accession #: NP_000007.1
• NCBI GenBank Nucleotide #: NM_000016.5
• UniProt Accession #: P11310; Q5T4U4; Q9NYF1
• Molecular Weight: 47,020 Da
• NCBI Official Full Name: medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform a
• NCBI Official Synonym Full Names: acyl-CoA dehydrogenase, C-4 to C-12 straight chain
• NCBI Official Symbol: ACADM
• NCBI Official Synonym Symbols: MCAD; ACAD1; MCADH
• NCBI Protein Information: medium-chain specific acyl-CoA dehydrogenase, mitochondrial; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• UniProt Protein Name: Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
• Protein Family: Medium-chain specific acyl-CoA dehydrogenase
• UniProt Gene Name: ACADM
• UniProt Synonym Gene Names: MCAD
• UniProt Entry Name: ACADM_HUMAN

NCBI Description

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACADM: This enzyme is specific for acyl chain lengths of 4 to 16. Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD). It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy. Belongs to the acyl-CoA dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.3.8.7; Mitochondrial; Carbohydrate Metabolism - propanoate; Oxidoreductase; Lipid Metabolism - fatty acid; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Other Amino Acids Metabolism - beta-alanine

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: mitochondrion; axon; mitochondrial matrix; nucleus

Molecular Function: acyl-CoA dehydrogenase activity; identical protein binding; FAD binding

Biological Process: carnitine metabolic process, CoA-linked; fatty acid beta-oxidation; medium-chain fatty acid catabolic process; cellular lipid metabolic process; medium-chain fatty acid metabolic process; fatty acid beta-oxidation using acyl-CoA dehydrogenase; carnitine biosynthetic process

Disease: Acyl-coa Dehydrogenase, Medium-chain, Deficiency Of