Rabbit anti-Human ABHD12 Polyclonal Antibody | anti-ABHD12 antibody

ABHD12, NT (Abhydrolase Domain-containing Protein 12, 2-arachidonoylglycerol Hydrolase, Monoacylglycerol Lipase ABHD12, C20orf22) (FITC)

Cat. Num. AAA6460874

• Gene Names: ABHD12; PHARC; ABHD12A; BEM46L2; C20orf22; dJ965G21.2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ABHD12; Polyclonal Antibody; NT (Abhydrolase Domain-containing Protein 12; 2-arachidonoylglycerol Hydrolase; Monoacylglycerol Lipase ABHD12; C20orf22) (FITC); anti-ABHD12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ABHD12.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ABHD12 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ABHD12 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ABHD12 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane). ABHD12 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with ABHD12 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ABHD12 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ABHD12 antibody

Antibodies; Enzymes; Lipase

References

1. Bechtel S., et.al., BMC Genomics 8:399-399(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 109689718
• NCBI GeneID: 26090
• NCBI Accession #: NP_001035937.1
• NCBI GenBank Nucleotide #: NM_001042472.2
• UniProt Accession #: Q8N2K0; Q5T710; Q5T711; Q96CR1; Q9BX05; Q9NPX7; Q9UFV6; A6NED4; A6NJ90; A8K450; B4DE71
• Molecular Weight: 45,097 Da
• NCBI Official Full Name: monoacylglycerol lipase ABHD12 isoform a
• NCBI Official Synonym Full Names: abhydrolase domain containing 12
• NCBI Official Symbol: ABHD12
• NCBI Official Synonym Symbols: PHARC; ABHD12A; BEM46L2; C20orf22; dJ965G21.2
• NCBI Protein Information: monoacylglycerol lipase ABHD12; 2-arachidonoylglycerol hydrolase; abhydrolase domain-containing protein 12
• UniProt Protein Name: Monoacylglycerol lipase ABHD12
• Protein Family: Monoacylglycerol lipase
• UniProt Gene Name: ABHD12
• UniProt Synonym Gene Names: C20orf22
• UniProt Entry Name: ABD12_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the hydrolysis of 2-arachidonoyl glycerol (2-AG), the main endocannabinoid lipid transmitter that acts on cannabinoid receptors, CB1 and CB2. The endocannabinoid system is involved in a wide range of physiological processes, including neurotransmission, mood, appetite, pain appreciation, addiction behavior, and inflammation. Mutations in this gene are associated with the neurodegenerative disease, PHARC (polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataract), resulting from an inborn error of endocannabinoid metabolism. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jan 2011]

Uniprot Description

ABHD12: Has 2-arachidonoylglycerol hydrolase activity. May be a regulator of endocannabinoid signaling pathways. Defects in ABHD12 are the cause of polyneuropathy hearing loss ataxia retinitis pigmentosa and cataract (PHARC). PHARC is a slowly progressive neurologic disorder with a variable phenotype resembling Refsum disease. Clinical features include sensorineural hearing loss, visual problems related to cataracts, retinitis pigmentosa, pes cavus, ataxic and/or spastic gait disturbances with a progressive sensorimotor peripheral neuropathy. Other features include hyporeflexia, hyperreflexia, extensor plantar responses. Belongs to the serine esterase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.23; Hydrolase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: integral to membrane

Molecular Function: acylglycerol lipase activity

Biological Process: phosphatidylserine catabolic process; adult walking behavior; acylglycerol catabolic process

Disease: Polyneuropathy, Hearing Loss, Ataxia, Retinitis Pigmentosa, And Cataract