Rabbit anti-Rat Interferon gamma Polyclonal Antibody | anti-IFNG antibody

Interferon gamma (IFG, IFI, IFN Gamma, IFN Immune, IFN, immune, IFN-gamma, IFNG, IFNG_HUMAN, Immune Interferon, Interferon Gamma Precursor, Macrophage Activating Factor, MAF, T Cell Interferon, Type II Interferon) (Biotin)

Cat. Num. AAA6460729

• Gene Names: Ifng; IFNG2
• Reactivity: Rat
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Interferon gamma; Polyclonal Antibody; Interferon gamma (IFG; IFI; IFN Gamma; IFN Immune; IFN; immune; IFN-gamma; IFNG; IFNG_HUMAN; Immune Interferon; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon) (Biotin); anti-IFNG antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Interferon gamma.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

• Applicable Applications for anti-IFNG antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions.; ELISA: 0.1-0.5ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to rat Interferon gamma expressed in E. coli. (Position: Q23-C156). Species sequence homology: human (38%) and mouse (86%)
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Anti-Interferon gamma antibody, All lanes: Anti-Interferon gamma at 0.5ug/ml WB: Recombinant Rat Interferon gamma Protein 0.5ng Predicted band size: 19KD Observed band size: 19KD)

Product Categories/Family for anti-IFNG antibody

Antibodies; Interferon

References

1. Nanba D, Higashiyama S (February 2004). "Dual intracellular signaling by proteolytic cleavage of membrane-anchored heparin-binding EGF-like growth factor". Cytokine Growth Factor Rev. 15(1): 13-9. 2. Jin K, Mao XO, Sun Y, Xie L, Jin L, Nishi E, Klagsbrun M, Greenberg DA (July 2002). "Heparin-binding epidermal growth factor-like growth factor: hypoxia-inducible expression in vitro and stimulation of neurogenesis in vitro and in vivo". J. Neurosci. 22(13): 5365-73. 3. Fen, Z., Dhadly, M. S., Yoshizumi, M., Hilkert, R. J., Quertermous, T., Eddy, R. L., Shows, T. B., Lee, M.-E. Structural organization and chromosomal assignment of the gene encoding the human heparin-binding epidermal growth factor-like growth factor/diphtheria toxin receptor. Biochemistry 32: 7932-7938, 1993.

NCBI and Uniprot Product Information

• NCBI GI #: 20302055
• NCBI GeneID: 25712
• NCBI Accession #: NP_620235.1
• NCBI GenBank Nucleotide #: NM_138880.2
• UniProt Accession #: P01581
• Molecular Weight: 17,918 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: Ifng
• NCBI Official Synonym Symbols: IFNG2
• NCBI Protein Information: interferon gamma; IFN-gamma
• UniProt Protein Name: Interferon gamma
• Protein Family: Interferon
• UniProt Gene Name: Ifng
• UniProt Synonym Gene Names: IFN-gamma
• UniProt Entry Name: IFNG_RAT

NCBI Description

an immune molecule produced by T lymphocytes in response to mitogens or antigens [RGD, Feb 2006]

Uniprot Description

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.