Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Microcephalin (MCPH1) Standard Curve.)
Mouse Microcephalin (MCPH1) ELISA Kit | MCPH1 elisa kit
Mouse Microcephalin (MCPH1) ELISA Kit
Gene Names
Mcph1; MCT; BRIT1; 5430437K10Rik; D030046N04Rik; Tg(HLA-A2.1)1Enge
Reactivity
Mouse
Synonyms
Microcephalin (MCPH1); Mouse Microcephalin (MCPH1) ELISA Kit; MCPH1; BRIT1; FLJ12847; MCT; BRCT-repeat inhibitor of TERT expression 1; microcephalin; microcephaly; primary autosomal recessive 1; MCPH1 elisa kit
Reactivity
Mouse
Specificity
Mouse Microcephalin (MCPH1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MCPH1. No significant cross-reactivity or interference between Mouse MCPH1 and analogues was observed.
Sequence Length
822
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Microcephalin (MCPH1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Microcephalin (MCPH1) Standard Curve.)
Related Product Information for MCPH1 elisa kit
Description: This Mouse Microcephalin (MCPH1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCPH1 in samples. An antibody specific for MCPH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCPH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCPH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCPH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MCPH1 encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in MCPH1 have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described.
Background: MCPH1 encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in MCPH1 have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microcephalin
NCBI Official Synonym Full Names
microcephaly, primary autosomal recessive 1
NCBI Official Symbol
Mcph1
NCBI Official Synonym Symbols
MCT; BRIT1; 5430437K10Rik; D030046N04Rik; Tg(HLA-A2.1)1Enge
NCBI Protein Information
microcephalin
UniProt Protein Name
Microcephalin
Protein Family
UniProt Gene Name
Mcph1
UniProt Entry Name
MCPH1_MOUSE
Research Articles on MCPH1
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Product Notes
The Mouse MCPH1 mcph1 (Catalog #AAA9717704) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717704 ELISA Kit recognizes Mouse MCPH1. It is sometimes possible for the material contained within the vial of "Microcephalin (MCPH1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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