Rabbit anti-Human NUP210 Polyclonal Antibody | anti-NUP210 antibody

NUP210, NT (NUP210, KIAA0906, Nuclear pore membrane glycoprotein 210, Nuclear envelope pore membrane protein POM 210, Nucleoporin Nup210, Pore membrane protein of 210kD)

Cat. Num. AAA645881

• Gene Names: NUP210; GP210; POM210
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NUP210; Polyclonal Antibody; NT (NUP210; KIAA0906; Nuclear pore membrane glycoprotein 210; Nuclear envelope pore membrane protein POM 210; Nucleoporin Nup210; Pore membrane protein of 210kD); Anti -NUP210; anti-NUP210 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-NUP210 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: NUP210 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209~239 amino acids from the N-terminal region of human NUP210.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of T47D cell line lysates (35ug/lane) using MBS645881. Arrow indicates NUP210 was detected using MBS645881.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human lung carcinoma using MBS645881 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MBS645881 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of T47D cells using MBS645881 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NUP210 antibody

NUP210 nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex.

Product Categories/Family for anti-NUP210 antibody

Antibodies; Abs to KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 27477134
• NCBI GeneID: 23225
• NCBI Accession #: NP_079199.2
• NCBI GenBank Nucleotide #: NM_024923.3
• UniProt Accession #: Q8TEM1; O94980; Q6NXG6; Q8NBJ1; Q9H6C8; Q9UFP3; A6NN56
• Molecular Weight: 205,111 Da
• NCBI Official Full Name: nuclear pore membrane glycoprotein 210
• NCBI Official Synonym Full Names: nucleoporin 210kDa
• NCBI Official Symbol: NUP210
• NCBI Official Synonym Symbols: GP210; POM210
• NCBI Protein Information: nuclear pore membrane glycoprotein 210; nucleoporin Nup210; nuclear pore protein gp210; pore membrane protein of 210 kDa; nuclear envelope pore membrane protein POM 210
• UniProt Protein Name: Nuclear pore membrane glycoprotein 210
• Protein Family: Nuclear pore membrane glycoprotein
• UniProt Gene Name: NUP210
• UniProt Synonym Gene Names: KIAA0906; Nuclear pore protein gp210; POM210
• UniProt Entry Name: PO210_HUMAN

NCBI Description

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3. [provided by RefSeq, Jul 2013]

Uniprot Description

Function: Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity. Ref.8

Subunit structure: Forms dimers and possibly higher-order oligomers

By similarity.

Subcellular location: Nucleus › nuclear pore complex. Nucleus membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein Ref.7.

Tissue specificity: Ubiquitous expression, with highest levels in lung, liver, pancreas, testis, and ovary, intermediate levels in brain, kidney, and spleen, and lowest levels in heart and skeletal muscle. Ref.4

Post-translational modification: N-glycosylated, but not all potential glycosylation sites may be used. Contains high-mannose type oligosaccharides

By similarity.Phosphorylated at Ser-1881 in mitosis specifically; not phosphorylated in interphase

By similarity.

Miscellaneous: Recognized by antinuclear autoantibodies in primary biliary cirrhosis.Knockdown of NUP210 causes nuclear membranes to accumulate aberrant structures termed twinned and fusion-arrested membranes and nuclear pore complex to cluster. Induces cell death and chromatin disruptions.

Sequence similarities: Belongs to the NUP210 family.

Sequence caution: The sequence BAB15332.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC11688.1 differs from that shown. Reason: Erroneous initiation.