Rabbit anti-Human, Mouse LMX1A Polyclonal Antibody | anti-LMX1A antibody

LMX1A (LIM Homeobox Transcription Factor 1-alpha, LIM/Homeobox Protein 11, LMX-11, LIM/Homeobox Protein LMX1A, LMX1A) (MaxLight 650)

Cat. Num. AAA6457028

• Gene Names: LMX1A; LMX1; LMX1.1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: LMX1A; Polyclonal Antibody; LMX1A (LIM Homeobox Transcription Factor 1-alpha; LIM/Homeobox Protein 11; LMX-11; LIM/Homeobox Protein LMX1A; LMX1A) (MaxLight 650); anti-LMX1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LMX1A. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-LMX1A antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 303-337 in the C-terminal region of human LMX1A.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in human liver lysates (20ug) using 302536 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 43kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-LMX1A antibody

Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae (By similarity).

Product Categories/Family for anti-LMX1A antibody

Antibodies; Transcription Factors

References

1. Thameem F., et al. Gene 290:217-225(2002). 2. Ota T., et al. Nat. Genet. 36:40-45(2004). 3. Gregory S.G., et al. Nature 441:315-321(2006). 4. Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 28893581
• NCBI GeneID: 4009
• NCBI Accession #: NP_796372
• NCBI GenBank Nucleotide #: NM_177398.3
• UniProt Accession #: Q8TE12; Q0VDB5; Q5VWG4; Q8TE11; B3KXP6
• Molecular Weight: 42 kDa
• NCBI Official Full Name: LIM homeobox transcription factor 1-alpha
• NCBI Official Synonym Full Names: LIM homeobox transcription factor 1, alpha
• NCBI Official Symbol: LMX1A
• NCBI Official Synonym Symbols: LMX1; LMX1.1
• NCBI Protein Information: LIM homeobox transcription factor 1-alpha; LIM/homeobox protein 1.1
• UniProt Protein Name: LIM homeobox transcription factor 1-alpha
• Protein Family: LIM homeobox transcription factor
• UniProt Gene Name: LMX1A
• UniProt Synonym Gene Names: LMX-1.1
• UniProt Entry Name: LMX1A_HUMAN

NCBI Description

This gene encodes a homeodomain and LIM-domain containing protein. The encoded protein is a transcription factor that acts as a positive regulator of insulin gene transcription. This gene also plays a role in the development of dopamine producing neurons during embryogenesis. Mutations in this gene are associated with an increased risk of developing Parkinson's disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

LMX1A: Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q24.1

Cellular Component: nucleus

Molecular Function: zinc ion binding; sequence-specific DNA binding

Biological Process: axon guidance; negative regulation of neuron differentiation; central nervous system neuron differentiation; dentate gyrus development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; midbrain development; cerebellum development; regulation of cell growth