Rabbit anti-Human, Mouse AGER Polyclonal Antibody | anti-AGER antibody

AGER, NT (AGER, RAGE, Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products)

Cat. Num. AAA645609

• Gene Names: AGER; RAGE
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: AGER; Polyclonal Antibody; NT (AGER; RAGE; Advanced glycosylation end product-specific receptor; Receptor for advanced glycosylation end products); Anti -AGER; anti-AGER antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AGER. Species
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-AGER antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Western Blot: 1:1000; Immunohistochemistry (FFPE): 1:10-1:50; Flow Cytometry (Indirect): 1:10-1:50; ; Optimal dilutions to be determined by the researcher
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment ofresidues within amino acids 24-52 in the N-terminal region ofhuman AGER.
• Cellular Localization: Cell membrane; Single-passtype I membrane protein
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse lung tissue lysates (35ug/lane) using MBS645609. AGER (arrow) was detected using MBS645609.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS645609 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometry of U251 cells using MBS645609 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-AGER antibody

AGER encodes a member of the immunoglobulin superfamily of cell surface molecules. It is a receptor for various molecules, including the amyloidogenic form of serum amyloid A, amyloid-beta protein, members of the S100/calgranulin superfamily and advanced glycation end products. The gene lies within the major histocompatibility complex (MHC) class III region on chromosome 6.

Product Categories/Family for anti-AGER antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 332800988
• NCBI GeneID: 177
• NCBI Accession #: NP_001193895.1
• NCBI GenBank Nucleotide #: NM_001206966.1
• UniProt Accession #: Q15109; Q15279; Q3L1R4; Q3L1R5; Q3L1R6; Q3L1R7; Q3L1R8; Q86SN1; A2BFI7; A6NKF0; A7Y2U9; B0V176
• NCBI Official Full Name: advanced glycosylation end product-specific receptor isoform 6
• NCBI Official Synonym Full Names: advanced glycosylation end product-specific receptor
• NCBI Official Symbol: AGER
• NCBI Official Synonym Symbols: RAGE
• NCBI Protein Information: advanced glycosylation end product-specific receptor; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• Protein Family: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: AGER
• UniProt Synonym Gene Names: RAGE
• UniProt Entry Name: RAGE_HUMAN

NCBI Description

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]

Uniprot Description

Function: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling

By similarity. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides. Ref.13 Ref.14 Ref.16 Ref.18

Subunit structure: Interacts with S100A1 and APP

By similarity. Interacts with S100B, S100A12 and S100A14. Ref.12 Ref.14 Ref.16 Ref.17

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Tissue specificity: Endothelial cells.

Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.