Western Blot (WB)
(IARS MaxPab rabbit polyclonal antibody. Western Blot analysis of IARS expression in Jurkat.)
Rabbit anti-Human IARS Polyclonal Antibody | anti-IARS antibody
IARS (Isoleucyl-tRNA Synthetase, FLJ20736, IARS1, ILRS, PRO0785) (HRP)
Gene Names
IARS; IRS; ILRS; IARS1; ILERS; PRO0785
Reactivity
Human
Applications
Western Blot
Purity
Purified
Synonyms
IARS; Polyclonal Antibody; IARS (Isoleucyl-tRNA Synthetase; FLJ20736; IARS1; ILRS; PRO0785) (HRP); Isoleucyl-tRNA Synthetase; PRO0785; anti-IARS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human IARS.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-IARS antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
IARS (AAH65552.1, 1aa-1055aa) full-length human protein.
Immunogen Sequence
MESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVAEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Western Blot (WB)
(IARS MaxPab rabbit polyclonal antibody. Western Blot analysis of IARS expression in Jurkat.)
Western Blot (WB)
(IARS MaxPab rabbit polyclonal antibody. Western Blot analysis of IARS expression in Jurkat.)
Western Blot (WB)
(Western Blot analysis of IARS expression in transfected 293T cell line by IARS MaxPab polyclonal antibody.Lane 1: IARS transfected lysate(120.60 KDa).Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of IARS expression in transfected 293T cell line by IARS MaxPab polyclonal antibody.Lane 1: IARS transfected lysate(120.60 KDa).Lane 2: Non-transfected lysate.)
Product Categories/Family for anti-IARS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
144 kDa (MW of target protein)
NCBI Official Full Name
IARS protein
NCBI Official Synonym Full Names
isoleucyl-tRNA synthetase
NCBI Official Symbol
IARS
NCBI Official Synonym Symbols
IRS; ILRS; IARS1; ILERS; PRO0785
NCBI Protein Information
isoleucine--tRNA ligase, cytoplasmic
NCBI Description
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012]
Research Articles on IARS
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Product Notes
The IARS (Catalog #AAA6451128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IARS (Isoleucyl-tRNA Synthetase, FLJ20736, IARS1, ILRS, PRO0785) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IARS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IARS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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