Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Formalin Fixed Paraffin Embedded (FFPE) human prostate tissue (Fig. 1) and mouse cerebellum tissue (Fig. 2) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution Anti-PDE9A. Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. Positive granular cytoplasmic staining was observed in human prostate glandular epithelial cells of human prostate tissue. Positive cytoplasmic staining was also observed in possible golgi type II and basket cells in the Purkinje and molecular layers of mouse cerebellum.)
Rabbit Phosphodiesterase 9A Polyclonal Antibody | anti-PDE9A antibody
Phosphodiesterase 9A (PDE9A, High Affinity cGMP-specific 3',5'-cyclic Phosphodiesterase 9A, FLJ90181, HSPDE9A2) (MaxLight 490)
Gene Names
PDE9A; FLJ90181; HSPDE9A2; PDE9A
Reactivity
Human, Rhesus Macaque, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Phosphodiesterase 9A; Polyclonal Antibody; Phosphodiesterase 9A (PDE9A; High Affinity cGMP-specific 3'; 5'-cyclic Phosphodiesterase 9A; FLJ90181; HSPDE9A2) (MaxLight 490); anti-PDE9A antibody
Host
Rabbit
Reactivity
Human, Rhesus Macaque, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PDE9A. Species Crossreactivity: mouse and rhesus macaque.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-PDE9A antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:100
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Linear peptide corresponding to human PDE9A, conjugated to KLH. Species sequence homology: rat and chimpanzee (100%)
Conjugate
MaxLight490
Control
Human spleen tissue lysate
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Formalin Fixed Paraffin Embedded (FFPE) human prostate tissue (Fig. 1) and mouse cerebellum tissue (Fig. 2) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution Anti-PDE9A. Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. Positive granular cytoplasmic staining was observed in human prostate glandular epithelial cells of human prostate tissue. Positive cytoplasmic staining was also observed in possible golgi type II and basket cells in the Purkinje and molecular layers of mouse cerebellum.)
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Formalin Fixed Paraffin Embedded (FFPE) human prostate tissue (Fig. 1) and mouse cerebellum tissue (Fig. 2) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution Anti-PDE9A. Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. Positive granular cytoplasmic staining was observed in human prostate glandular epithelial cells of human prostate tissue. Positive cytoplasmic staining was also observed in possible golgi type II and basket cells in the Purkinje and molecular layers of mouse cerebellum.)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Human spleen tissue lysate was probed with Anti-PDE9A (0.5ug/ml). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PDEA9 (~75kD).)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Human spleen tissue lysate was probed with Anti-PDE9A (0.5ug/ml). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PDEA9 (~75kD).)
Product Categories/Family for anti-PDE9A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform b [Homo sapiens]
NCBI Official Synonym Full Names
phosphodiesterase 9A
NCBI Official Symbol
PDE9A
NCBI Official Synonym Symbols
FLJ90181; HSPDE9A2; PDE9A
UniProt Protein Name
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
Protein Family
UniProt Gene Name
PDE9A
UniProt Entry Name
PDE9A_HUMAN
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Product Notes
The PDE9A pde9a (Catalog #AAA6444738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phosphodiesterase 9A (PDE9A, High Affinity cGMP-specific 3',5'-cyclic Phosphodiesterase 9A, FLJ90181, HSPDE9A2) (MaxLight 490) reacts with Human, Rhesus Macaque, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphodiesterase 9A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PDE9A pde9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphodiesterase 9A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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