Western Blot (WB)
(Western blot analysis of Villin-1 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). VIL1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse Villin Polyclonal Antibody
Villin, NT (VIL, Villin 1, Villin-1, VIL1, D2S1471, OTTHUMP00000164145)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
Villin; Polyclonal Antibody; NT (VIL; Villin 1; Villin-1; VIL1; D2S1471; OTTHUMP00000164145); Anti -Villin; anti-Villin antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human VILLIN1. Species Crossreactivity: mouse.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-Villin antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in ELISA, Western Blot, Flow Cytometry, and Immunohistochemistry.
Dilution: Western Blot: 1:50-1:100
ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
Flow Cytometry: 1:10-1:50
Dilution: Western Blot: 1:50-1:100
ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
Flow Cytometry: 1:10-1:50
Immunogen
Synthetic peptide selected from the N-terminal region of human VILLIN1 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of Villin-1 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). VIL1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of Villin-1 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). VIL1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human colon carcinoma reacted with Villin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human colon carcinoma reacted with Villin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of WiDr cells using Villin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of WiDr cells using Villin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-Villin antibody
Villin-1 is a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments.
Product Categories/Family for anti-Villin antibody
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]
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Product Notes
The Villin (Catalog #AAA644207) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Villin, NT (VIL, Villin 1, Villin-1, VIL1, D2S1471, OTTHUMP00000164145) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Villin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Western Blot, Flow Cytometry, and Immunohistochemistry. Dilution: Western Blot: 1:50-1:100 ELISA: 1:1,000 Immunohistochemistry: 1:50-1:100 Flow Cytometry: 1:10-1:50. Researchers should empirically determine the suitability of the Villin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Villin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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