Western Blot (WB)
(Western blot analysis of extracts of MCF7 cells tissue, using PRKCI antibody.)
Rabbit PRKCI Polyclonal Antibody | anti-PRKCI antibody
PRKCI (Protein Kinase C iota Type, DXS1179E, MGC26534, PKCI, nPKC-iota) (AP)
WB: 1:500-1:2000
IP: 1:50-1:200
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of extracts of MCF7 cells tissue, using PRKCI antibody.)
Western Blot (WB)
(Western blot analysis of extracts of MCF7 cells tissue, using PRKCI antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]
Uniprot Description
PKCI: an AGC protein kinase of the PKC family. A calcium-independent, phospholipid-dependent atypical protein kinase C. Is not activated by phorbolesters or diaglycerol. May play role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets.
Protein type: Oncoprotein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Cell adhesion; EC 2.7.11.13; Cell development/differentiation; Motility/polarity/chemotaxis; Tumor suppressor; AGC group; PKC family; Iota subfamily
Chromosomal Location of Human Ortholog: 3q26.3
Cellular Component: Golgi membrane; microtubule cytoskeleton; intercellular bridge; tight junction; protein complex; leading edge; cytoplasm; apical plasma membrane; plasma membrane; cytosol; nucleus; endosome
Molecular Function: protein serine/threonine kinase activity; protein domain specific binding; protein binding; protein kinase C activity; metal ion binding; phospholipid binding; ATP binding; protein kinase activity
Biological Process: intercellular junction assembly and maintenance; secretion; cell migration; nerve growth factor receptor signaling pathway; establishment of apical/basal cell polarity; actin filament organization; cytoskeleton organization and biogenesis; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; establishment and/or maintenance of epithelial cell polarity; vesicle-mediated transport; positive regulation of glucose import; eye photoreceptor cell development; cellular response to insulin stimulus; negative regulation of neuron apoptosis; Golgi vesicle budding; protein targeting to membrane; negative regulation of apoptosis
Research Articles on PRKCI
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Product Notes
The PRKCI prkci (Catalog #AAA6433008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRKCI (Protein Kinase C iota Type, DXS1179E, MGC26534, PKCI, nPKC-iota) (AP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRKCI can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP). IHC: 1:50-1:200 WB: 1:500-1:2000 IP: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PRKCI prkci for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKCI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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