Rabbit anti-Human MARCKS Polyclonal Antibody | anti-MARCKS antibody

MARCKS, Phosphorylated (S170) (Myristoylated Alanine-rich C-kinase Substrate, MACS, 80K-L, PKCSL, PRKCSL) (HRP)

Cat. Num. AAA6426071

• Gene Names: MARCKS; MACS; 80K-L; PKCSL; PRKCSL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: MARCKS; Polyclonal Antibody; Phosphorylated (S170) (Myristoylated Alanine-rich C-kinase Substrate; MACS; 80K-L; PKCSL; PRKCSL) (HRP); anti-MARCKS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MARCKS, phosphorylated S170.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-MARCKS antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding S170 of human MARCKS.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from MCF cells, using phospho-MARCKS-S170 antibody.)

Product Categories/Family for anti-MARCKS antibody

Antibodies; Protein Kinase Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 153070260
• NCBI GeneID: 4082
• NCBI Accession #: NP_002347.5
• NCBI GenBank Nucleotide #: NM_002356.5
• UniProt Accession #: P29966; Q2LA83; Q5TDB7; E1P560
• Molecular Weight: 31,555 Da
• NCBI Official Full Name: myristoylated alanine-rich C-kinase substrate
• NCBI Official Synonym Full Names: myristoylated alanine-rich protein kinase C substrate
• NCBI Official Symbol: MARCKS
• NCBI Official Synonym Symbols: MACS; 80K-L; PKCSL; PRKCSL
• NCBI Protein Information: myristoylated alanine-rich C-kinase substrate; phosphomyristin; protein kinase C substrate, 80 kDa protein, light chain; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
• UniProt Protein Name: Myristoylated alanine-rich C-kinase substrate
• Protein Family: MARCKS-related protein
• UniProt Gene Name: MARCKS
• UniProt Synonym Gene Names: MACS; PRKCSL; MARCKS; 80K-L protein; PKCSL
• UniProt Entry Name: MARCS_HUMAN

NCBI Description

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

MARCKS: the most prominent cellular substrate of protein kinase C. Binds calmodulin, actin, and synapsin. A filamentous actin cross-linking protein. Critical for normal brain development. Phosphorylation by PKC displaces it from the plasma membrane, inhibiting F-actin cross-linking activity. Plays a role in growth cone adhesion: its nonphosphorylated form stabilizes integrin-mediated adhesions, and its phosphorylation-triggered release from adhesions causes localized growth cone detachment. Highly expressed in immature T and B lymphocytes: its expression is highest in CD4-8- T cells and B-cell blasts generated by antigen receptor signaling. Its expression and phosphorylation are closely linked to microglial activation processes involved in pathological conditions such as neuroinflammation and neurodegeneration.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 6q22.2

Cellular Component: germinal vesicle; centrosome; focal adhesion; plasma membrane; cell cortex; actin cytoskeleton

Molecular Function: calmodulin binding; actin filament binding; protein kinase C binding

Biological Process: energy reserve metabolic process; regulation of insulin secretion