Rabbit HCFC1 Polyclonal Antibody | anti-HCFC1 antibody

HCFC1 (Host Cell Factor C1, CFF, HCF1, HFC1, MRX3, VCAF, HCF-1) (FITC)

Cat. Num. AAA6419932

• Gene Names: HCFC1; CFF; HCF1; HFC1; MRX3; VCAF; HCF-1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: HCFC1; Polyclonal Antibody; HCFC1 (Host Cell Factor C1; CFF; HCF1; HFC1; MRX3; VCAF; HCF-1) (FITC); anti-HCFC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes HCFC1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-HCFC1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human HCFC1.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of HEK-293 cell lines, using HCFC1 antibody.)

Product Categories/Family for anti-HCFC1 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 98986457
• NCBI GeneID: 3054
• NCBI Accession #: NP_005325.2
• NCBI GenBank Nucleotide #: NM_005334.2
• UniProt Accession #: P51610; Q6P4G5; AAB27583
• Molecular Weight: 208,732 Da
• NCBI Official Full Name: host cell factor 1
• NCBI Official Synonym Full Names: host cell factor C1 (VP16-accessory protein)
• NCBI Official Symbol: HCFC1
• NCBI Official Synonym Symbols: CFF; HCF1; HFC1; MRX3; VCAF; HCF-1
• NCBI Protein Information: host cell factor 1; HCF; C1 factor; VP16 accessory protein
• UniProt Protein Name: Host cell factor 1
• Protein Family: Host cell factor
• UniProt Gene Name: HCFC1
• UniProt Synonym Gene Names: HCF1; HFC1; HCF; HCF-1
• UniProt Entry Name: HCFC1_HUMAN

NCBI Description

This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HCFC1: Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; cell soma; mitochondrion; membrane; cytoplasm; nucleus; histone acetyltransferase complex

Molecular Function: identical protein binding; protein binding; transcription coactivator activity; chromatin binding; histone acetyltransferase activity (H4-K16 specific); transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; establishment and/or maintenance of chromatin architecture; protein stabilization; regulation of transcription, DNA-dependent; organelle organization and biogenesis; reactivation of latent virus; regulation of protein complex assembly; positive regulation of cell cycle; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell cycle

Disease: Methylmalonic Acidemia And Homocysteinemia, Cblx Type