Goat PHD3 Polyclonal Antibody | anti-LOC100170696 antibody

PHD3 (Egl Nine Homolog 3, HPH-1, Hypoxia-inducible Factor Prolyl Hydroxylase 3, HIF-PH3, HIF-prolyl Hydroxylase 3, HPH-3, Prolyl Hydroxylase Domain-containing Protein 3, EGLN3)

Cat. Num. AAA641622

• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PHD3; Polyclonal Antibody; PHD3 (Egl Nine Homolog 3; HPH-1; Hypoxia-inducible Factor Prolyl Hydroxylase 3; HIF-PH3; HIF-prolyl Hydroxylase 3; HPH-3; Prolyl Hydroxylase Domain-containing Protein 3; EGLN3); Anti -PHD3 (Egl Nine Homolog 3; anti-LOC100170696 antibody

• Host: Goat
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human EGLN3. Species sequence homology: Bovine, canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-LOC100170696 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:8000; Western Blot: 0.1-0.3ug/ml, observed in human skeletal muscle lysates on ~30kD bands
• Immunogen: Synthetic peptide corresponding to C-RLGKYYVKERSK, from human EGLN3, at the internal region of the protein (NP_071356.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((0.1ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-LOC100170696 antibody

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation.

Product Categories/Family for anti-LOC100170696 antibody

Antibodies; Abs to Hypoxia

NCBI and Uniprot Product Information

• NCBI GI #: 351725177
• NCBI GeneID: 100170696
• NCBI Accession #: NP_001237851.1
• NCBI GenBank Nucleotide #: NM_001250922.1
• NCBI Official Full Name: PHD3
• NCBI Official Symbol: LOC100170696
• NCBI Protein Information: PHD3