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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CST8 antibody.)

Rabbit anti-Human CST8 Polyclonal Antibody | anti-CST8 antibody

CST8 (Cystatin 8, CRES) (PE)

Gene Names
CST8; CRES; CTES5
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
CST8; Polyclonal Antibody; CST8 (Cystatin 8; CRES) (PE); anti-CST8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CST8.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-CST8 antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
IF: 1:50-1:200
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human CST8.
Conjugate
PE
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CST8 antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CST8 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using CST8 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using CST8 antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using CST8 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using CST8 antibody. Blue: DAPI for nuclear staining.)
Product Categories/Family for anti-CST8 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
142
NCBI Official Full Name
Cystatin-8
NCBI Official Synonym Full Names
cystatin 8 (cystatin-related epididymal specific)
NCBI Official Symbol
CST8
NCBI Official Synonym Symbols
CRES; CTES5
NCBI Protein Information
cystatin-8; cystatin-related epididymal spermatogenic protein
UniProt Protein Name
Cystatin-8
Protein Family
UniProt Gene Name
CST8
UniProt Synonym Gene Names
CRES
UniProt Entry Name
CST8_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The encoded protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

CST8: Performs a specialized role during sperm development and maturation. Belongs to the cystatin family.

Protein type: Inhibitor; Secreted; Cell surface; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: cell surface; cytoplasm; extracellular region

Molecular Function: cysteine protease inhibitor activity

Research Articles on CST8

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Product Notes

The CST8 cst8 (Catalog #AAA6412756) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CST8 (Cystatin 8, CRES) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CST8 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CST8 cst8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CST8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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