Rabbit anti-Human CST8 Polyclonal Antibody | anti-CST8 antibody

CST8 (Cystatin 8, CRES) (HRP)

Cat. Num. AAA6412750

• Gene Names: CST8; CRES; CTES5
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: CST8; Polyclonal Antibody; CST8 (Cystatin 8; CRES) (HRP); anti-CST8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CST8.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-CST8 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human CST8.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CST8 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using CST8 antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using CST8 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-CST8 antibody

Antibodies; Protease Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 6225205
• NCBI GeneID: 10047
• UniProt Accession #: O60676; Q2M2X6
• Molecular Weight: 142
• NCBI Official Full Name: Cystatin-8
• NCBI Official Synonym Full Names: cystatin 8 (cystatin-related epididymal specific)
• NCBI Official Symbol: CST8
• NCBI Official Synonym Symbols: CRES; CTES5
• NCBI Protein Information: cystatin-8; cystatin-related epididymal spermatogenic protein
• UniProt Protein Name: Cystatin-8
• Protein Family: Cystatin
• UniProt Gene Name: CST8
• UniProt Synonym Gene Names: CRES
• UniProt Entry Name: CST8_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The encoded protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

CST8: Performs a specialized role during sperm development and maturation. Belongs to the cystatin family.

Protein type: Inhibitor; Secreted; Cell surface; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: cell surface; cytoplasm; extracellular region

Molecular Function: cysteine protease inhibitor activity