Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Stomach Tissue Set
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Stomach
Purity
Molecular Biology Grade
Synonyms
Tissue Set; BioAssay; DNA; RNA; Protein Adult Normal; Stomach; Protein; Mouse Adult Normal; Stomach tissue set
Purity/Purification
Molecular Biology Grade
Quality Control
• Genomic DNA:
1. The quality and purity of genomic DNA were tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5). 2. RNase treatment to ensure the RNA contamination is eliminated. 3. Hind III digestion test: genomic DNA was successfully digested by Hind III. 4. PCR test: A specific fragment of actin gene, about 800 bp, was successfully amplified from the genomic DNA.
• Total RNA:
1. The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5).
2. The RNA is treated by DNase I, and is tested as DNA free RNA by PCR.
3. cDNA synthesis is successfully performed by using this RNA as template.
Note:
1. Total RNA from some rare tissues and tumor tissues may not be treated by DNase I 2. To visualize the RNA images on agarose gel, we recommend this gel system be used (1%
agarose gel in 1X MOPS buffer with formaldehyde). MyBioSource is not responsible for customer's getting degraded RNA images from other gel systems, such as TAE gel, TBE gel, Urea gel, etc.
• Total Protein:
1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining, the pattern is consistent with each lot.
2. The isolated protein is Western analyzed by either GAPDH or -actin antibody, and the expression level is consistent with each lot.
Note: Due to the nature of tissues, some time even house keeping genes show weak signal or no signal in some tissues. So GAPDH and -actin cannot be guaranteed to show strong signals at the same time.
1. The quality and purity of genomic DNA were tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5). 2. RNase treatment to ensure the RNA contamination is eliminated. 3. Hind III digestion test: genomic DNA was successfully digested by Hind III. 4. PCR test: A specific fragment of actin gene, about 800 bp, was successfully amplified from the genomic DNA.
• Total RNA:
1. The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5).
2. The RNA is treated by DNase I, and is tested as DNA free RNA by PCR.
3. cDNA synthesis is successfully performed by using this RNA as template.
Note:
1. Total RNA from some rare tissues and tumor tissues may not be treated by DNase I 2. To visualize the RNA images on agarose gel, we recommend this gel system be used (1%
agarose gel in 1X MOPS buffer with formaldehyde). MyBioSource is not responsible for customer's getting degraded RNA images from other gel systems, such as TAE gel, TBE gel, Urea gel, etc.
• Total Protein:
1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining, the pattern is consistent with each lot.
2. The isolated protein is Western analyzed by either GAPDH or -actin antibody, and the expression level is consistent with each lot.
Note: Due to the nature of tissues, some time even house keeping genes show weak signal or no signal in some tissues. So GAPDH and -actin cannot be guaranteed to show strong signals at the same time.
Preparation and Storage
-70 degree C for Total RNA and long term protein, -20 degree C for short term protein, 2- 8 degree C for genomic DNA.
Shelf Life: 6 months from the date of receipt under proper storage condition.
Shelf Life: 6 months from the date of receipt under proper storage condition.
Related Product Information for Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Stomach tissue set
50ug RNA, 10ug genomic DNA, and 100ug protein per set
Tissue set is genomic DNA, RNA, and Protein isolated from the same piece of biomaterials, such as tissues or cells, Simultaneously. Tissue Set includes 50ug total RNA, 10ug genomic DNA, and 100ug protein. Tissue Set is particularly important for functional genomics and proteomics study, such as gene regulation, expression of genes and protein, among DNA, RNA, and protein.
This Tissue Set has almost the same high qualities as those products isolated separately by conventional methods. There are many commercialized kits and reagents for RNA isolation, though some of them can also isolate genomic DNA and protein at the same time, the qualities, especially of proteins, are not as good as the RNA quality. Comparing with the protein isolation only method, the yield is only 10-30%. This means many proteins are possibly missing, and therefore researchers seldom used proteins isolated by those kits and reagents, for protein expression profiling, protein quantitation, protein purification, and etc.
Tissue Set is ready to use.
Tissue set is genomic DNA, RNA, and Protein isolated from the same piece of biomaterials, such as tissues or cells, Simultaneously. Tissue Set includes 50ug total RNA, 10ug genomic DNA, and 100ug protein. Tissue Set is particularly important for functional genomics and proteomics study, such as gene regulation, expression of genes and protein, among DNA, RNA, and protein.
This Tissue Set has almost the same high qualities as those products isolated separately by conventional methods. There are many commercialized kits and reagents for RNA isolation, though some of them can also isolate genomic DNA and protein at the same time, the qualities, especially of proteins, are not as good as the RNA quality. Comparing with the protein isolation only method, the yield is only 10-30%. This means many proteins are possibly missing, and therefore researchers seldom used proteins isolated by those kits and reagents, for protein expression profiling, protein quantitation, protein purification, and etc.
Tissue Set is ready to use.
Similar Products
Product Notes
The Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Stomach (Catalog #AAA640963) is a Tissue Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Stomach, Tissue Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
