Rabbit ATP1B1 Polyclonal Antibody | anti-ATP1B1 antibody

ATP1B1 (Sodium/Potassium-transporting ATPase Subunit beta-1, ATP1B, ATP1B1, Beta 1 subunit of Na(+) K(+) ATPase, Na+/K+ ATPase beta 1 subunit) (APC)

Cat. Num. AAA6407687

• Gene Names: ATP1B1; ATP1B
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: ATP1B1; Polyclonal Antibody; ATP1B1 (Sodium/Potassium-transporting ATPase Subunit beta-1; ATP1B; Beta 1 subunit of Na(+) K(+) ATPase; Na+/K+ ATPase beta 1 subunit) (APC); anti-ATP1B1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes ATP1B1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-ATP1B1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human ATP1B1.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ATP1B1 antibody.)

Product Categories/Family for anti-ATP1B1 antibody

Antibodies; Enzymes; ATPase

NCBI and Uniprot Product Information

• NCBI GI #: 4502277
• NCBI GeneID: 481
• NCBI Accession #: NP_001668.1
• NCBI GenBank Nucleotide #: NM_001677.3
• UniProt Accession #: P05026; Q5TGZ3
• Molecular Weight: 34,893 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit beta-1
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, beta 1 polypeptide
• NCBI Official Symbol: ATP1B1
• NCBI Official Synonym Symbols: ATP1B
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-1; Beta 1-subunit of Na(+),K(+)-ATPase; Na, K-ATPase beta-1 polypeptide; adenosinetriphosphatase; sodium pump subunit beta-1; sodium-potassium ATPase subunit beta 1 (non-catalytic); sodium/potassium-depend
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit beta-1
• UniProt Gene Name: ATP1B1
• UniProt Synonym Gene Names: ATP1B
• UniProt Entry Name: AT1B1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]

Uniprot Description

ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: basolateral plasma membrane; apical plasma membrane; plasma membrane; caveola; sodium:potassium-exchanging ATPase complex; sarcolemma; vesicle

Molecular Function: protein binding; ATPase activator activity; sodium:potassium-exchanging ATPase activity; ATPase binding

Biological Process: cellular sodium ion homeostasis; protein stabilization; cellular potassium ion homeostasis; cellular calcium ion homeostasis; potassium ion import; positive regulation of ATPase activity; regulation of gene expression; response to hypoxia; blood coagulation; cell adhesion; leukocyte migration; transmembrane transport; cardiac muscle contraction

Disease: Hypertension, Essential