Rabbit ABAT Polyclonal Antibody | anti-ABAT antibody

ABAT (4-Aminobutyrate Aminotransferase, GABAT, NPD009, GABA-AT) (PE)

Cat. Num. AAA6404946

• Gene Names: ABAT; GABAT; NPD009; GABA-AT
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: ABAT; Polyclonal Antibody; ABAT (4-Aminobutyrate Aminotransferase; GABAT; NPD009; GABA-AT) (PE); anti-ABAT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes ABAT. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-ABAT antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human ABAT.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ABAT antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded mouse lung using ABAT antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human lung using ABAT antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using ABAT antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-ABAT antibody

Antibodies; Enzymes; Aminotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 48429239
• NCBI GeneID: 18
• UniProt Accession #: P80404; Q16260; Q8N5W2; Q96BG2; Q99800; A8K386
• Molecular Weight: 500
• NCBI Official Full Name: 4-aminobutyrate aminotransferase, mitochondrial
• NCBI Official Synonym Full Names: 4-aminobutyrate aminotransferase
• NCBI Official Symbol: ABAT
• NCBI Official Synonym Symbols: GABAT; NPD009; GABA-AT
• NCBI Protein Information: 4-aminobutyrate aminotransferase, mitochondrial; GABA transferase; GABA transaminase; GABA aminotransferase; 4-aminobutyrate transaminase; gamma-amino-N-butyrate transaminase; (S)-3-amino-2-methylpropionate transaminase
• UniProt Protein Name: 4-aminobutyrate aminotransferase, mitochondrial
• UniProt Gene Name: ABAT
• UniProt Synonym Gene Names: GABAT; GABA-AT; GABA transaminase; GABA-T
• UniProt Entry Name: GABT_HUMAN

NCBI Description

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ABAT: Catalyzes the conversion of gamma-aminobutyrate and L- beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine. Defects in ABAT are a cause of GABA transaminase deficiency (GABATD). The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Carbohydrate Metabolism - butanoate; EC 2.6.1.19; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - propanoate; Transferase; Other Amino Acids Metabolism - beta-alanine; EC 2.6.1.22

Chromosomal Location of Human Ortholog: 16p13.2

Cellular Component: neuron projection; mitochondrion; mitochondrial matrix; 4-aminobutyrate transaminase complex

Molecular Function: protein homodimerization activity; succinate-semialdehyde dehydrogenase binding; (S)-3-amino-2-methylpropionate transaminase activity; 4-aminobutyrate transaminase activity; pyridoxal phosphate binding

Biological Process: response to nicotine; copulation; response to drug; synaptic transmission; neurotransmitter catabolic process; response to ethanol; behavioral response to cocaine; neurotransmitter secretion; response to hypoxia; locomotory behavior; negative regulation of blood pressure; gamma-aminobutyric acid catabolic process; response to iron ion

Disease: Gaba-transaminase Deficiency