Western Blot (WB)
(PKN1 rabbit polyclonal antibody. Western Blot analysis of PKN1 expression in mouse testis.)
Rabbit anti-Human, Mouse PKN1 Polyclonal Antibody | anti-PKN1 antibody
PKN1 (Serine/Threonine-protein Kinase N1, Protease-activated Kinase 1, PAK-1, Protein Kinase C-like 1, Protein Kinase C-like PKN, Protein Kinase PKN-alpha, Protein-kinase C-related Kinase 1, Serine-threonine Protein Kinase N, PKN1, PAK1, PRK1, PRKCL1, MGC
Gene Names
PKN1; DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PKN1; Polyclonal Antibody; PKN1 (Serine/Threonine-protein Kinase N1; Protease-activated Kinase 1; PAK-1; Protein Kinase C-like 1; Protein Kinase C-like PKN; Protein Kinase PKN-alpha; Protein-kinase C-related Kinase 1; Serine-threonine Protein Kinase N; PAK1; PRK1; PRKCL1; MGC; anti-PKN1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PKN1. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Sequence Length
3160
Applicable Applications for anti-PKN1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human PKN1, aa1-942 (AAH40061.1).
Immunogen Sequence
MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDISNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(PKN1 rabbit polyclonal antibody. Western Blot analysis of PKN1 expression in mouse testis.)
Western Blot (WB)
(PKN1 rabbit polyclonal antibody. Western Blot analysis of PKN1 expression in mouse testis.)
Western Blot (WB)
(PKN1 rabbit polyclonal antibody. Western Blot analysis of PKN1 expression in K-562.)
Western Blot (WB)
(PKN1 rabbit polyclonal antibody. Western Blot analysis of PKN1 expression in K-562.)
Western Blot (WB)
(Western Blot analysis of PKN1 expression in transfected 293T cell line by PKN1 polyclonal antibody. Lane 1: PKN1 transfected lysate (103.9kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of PKN1 expression in transfected 293T cell line by PKN1 polyclonal antibody. Lane 1: PKN1 transfected lysate (103.9kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-PKN1 antibody
PKN, a member of the PKC subfamily of Ser/Thr protein kinases, can phosphorylate ribosomal protein S6. It mediates GTPase Rho dependent intracellular signalling. This protein is activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids This cytoplasmic protein is expressed ubiquitously, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Product Categories/Family for anti-PKN1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Homo sapiens protein kinase N1, mRNA
NCBI Official Synonym Full Names
protein kinase N1
NCBI Official Symbol
PKN1
NCBI Official Synonym Symbols
DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
NCBI Protein Information
serine/threonine-protein kinase N1
Protein Family
NCBI Description
The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Research Articles on PKN1
Similar Products
Product Notes
The PKN1 (Catalog #AAA6389596) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKN1 (Serine/Threonine-protein Kinase N1, Protease-activated Kinase 1, PAK-1, Protein Kinase C-like 1, Protein Kinase C-like PKN, Protein Kinase PKN-alpha, Protein-kinase C-related Kinase 1, Serine-threonine Protein Kinase N, PKN1, PAK1, PRK1, PRKCL1, MGC reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PKN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PKN1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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