Rabbit anti-Human PDYN Polyclonal Antibody | anti-PDYN antibody

PDYN (Proenkephalin-B, Beta-neoendorphin-dynorphin, Preprodynorphin, MGC26418) (HRP)

Cat. Num. AAA6388991

• Gene Names: PDYN; ADCA; PENKB; SCA23
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDYN; Polyclonal Antibody; PDYN (Proenkephalin-B; Beta-neoendorphin-dynorphin; Preprodynorphin; MGC26418) (HRP); anti-PDYN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDYN.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PDYN antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PDYN, aa1-254 (NP_077722.1).
• Immunogen Sequence: MAWQGLVLAACLLMFPSTTADCLSRCSLCAVKTQDGPKPINPLICSLQCQAALLPSEEWERCQSFLSFFTPSTLGLNDKEDLGSKSVGEGPYSELAKLSGSFLKELEKSKFLPSISTKENTLSKSLEEKLRGLSDGFREGAESELMRDAQLNDGAMETGTLYLAEEDPKEQVKRYGGFLRKYPKRSSEVAGEGDGDSMGHEDLYKRYGGFLRRIRPKLKWDNQKRYGGFLRRQFKVVTRSQEDPNAYSGELFDA
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PDYN expression in transfected 293T cell line by PDYN polyclonal antibody. Lane 1: PDYN transfected lysate (28.4kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PDYN antibody

Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin. Leumorphin has a typical opiod activity and may have anti-apoptotic effect.

Product Categories/Family for anti-PDYN antibody

Antibodies; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 13270473
• NCBI GeneID: 5173
• NCBI Accession #: NP_077722.1
• NCBI GenBank Nucleotide #: NM_024411
• UniProt Accession #: P01213; A8K0Q3
• Molecular Weight: 28,385 Da
• NCBI Official Full Name: proenkephalin-B preproprotein
• NCBI Official Synonym Full Names: prodynorphin
• NCBI Official Symbol: PDYN
• NCBI Official Synonym Symbols: ADCA; PENKB; SCA23
• NCBI Protein Information: proenkephalin-B
• UniProt Protein Name: Proenkephalin-B
• Protein Family: Proenkephalin
• UniProt Gene Name: PDYN
• UniProt Synonym Gene Names: Big Dyn; Dyn-A17; Dynorphin A; Dyn-B
• UniProt Entry Name: PDYN_HUMAN

NCBI Description

The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

PDYN: Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Defects in PDYN are the cause of spinocerebellar ataxia type 23 (SCA23). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA23 is an adult-onset autosomal dominant form characterized by slowly progressive gait and limb ataxia, with variable additional features, including peripheral neuropathy and dysarthria. Belongs to the opioid neuropeptide precursor family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: plasma membrane; extracellular region

Molecular Function: opioid peptide activity

Biological Process: synaptic transmission; neuropeptide signaling pathway

Disease: Spinocerebellar Ataxia 23