Rabbit anti-Human NELL1 Polyclonal Antibody | anti-NELL1 antibody

NELL1 (Protein Kinase C-binding Protein NELL1, NEL-like Protein 1, Nel-related Protein 1, NRP1) (FITC)

Cat. Num. AAA6386867

• Gene Names: NELL1; NRP1; IDH3GL
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NELL1; Polyclonal Antibody; NELL1 (Protein Kinase C-binding Protein NELL1; NEL-like Protein 1; Nel-related Protein 1; NRP1) (FITC); anti-NELL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NELL1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 2932

• Applicable Applications for anti-NELL1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human NELL1, aa1-810 (AAH69674.1).
• Immunogen Sequence: MPMDLILVVWFCVCTARTVVGFGMDPDLQMDIVTELDLVNTTLGVAQVSGMHNASKAFLFQDIEREIHAAPHVSEKLIQLFRNKSEFTILATVQQKPSTSGVILSIRELEHSYFELESSGLRDEIRYHYIHNGKPRTEALPYRMADGQWHKVALSVSASHLLLHVDCNRIYERVIDPPDTNLPPGINLWLGQRNQKHGLFKGIIQDGKIIFMPNGYITQCPNLNHTCPTCSDFLSLVQGIMDLQELLAKMTAKLNYAETRLSQLENCHCEKTCQVSGLLYRDQDSWVDGDHCRNCTCKSGAVECRRMSCPPLNCSPDSLPVHIAGQCCKVCRPKCIYGGKVLAEGQRILTKSCRECRGGVLVKITEMCPPLNCSEKDHILPENQCCRVCRGHNFCAEGPKCGENSECKNWNTKATCECKSGYISVQGDSAYCEDIDECAAKMHYCHANTVCVNLPGLYRCDCVPGYIRVDDFSCTEHDECGSGQHNCDENAICTNTVQGHSCTCKPGYVGNGTICRAFCEEGCRYGGTCVAPNKCVCPSGFTGSHCEKDIDECSEGIIECHNHSRCVNLPGWYHCECRSGFHDDGTYSLSGESCIDIDECALRTHTCWNDSACINLAGGFDCLCPSGPSCSGDCPHEGGLKHNGQVWTLKEDRCSVCSCKDGKIFCRRTACDCQNPSADLFCCPECDTRVTSQCLDQNGHKLYRSGDNWTHSCQQCRCLEGEVDCWPLTCPNLSCEYTAILEGECCPRCVSDPCLADNITYDIRKTCLDSYGVSRLSGSVWTMAGSPCTTCKCKNGRVCCSVDFECLQNN
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of NELL1 expression in transfected 293T cell line by NELL1 polyclonal antibody. Lane 1: NELL1 transfected lysate (89.6kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-NELL1 antibody

NELL1 (neural EGF-like like protein 1) is an 89kD (predicted) member of the EGF-like domain containing family, Laminin G/NTSP1/Pentraxin gene superfamily of molecules. When secreted, NELL1 exists as a phosphoglycoprotein that can add as much as 50kD to the calculated MW. NELL1 has restricted expression, being limited to pre-B cells and osteoblasts, where it apparently promotes osteoblast maturation and bone formation. In tumors, it is found in neuroblastoma-derived cells. NELL1 is both secreted and retained intracellularly where it is phosphorylated by PKC. The human NELL1 precursor is 810aa in length. It contains a16aa signal sequence plus a 794aa mature region. The mature region possesses an N-terminal TSP domain (aa81-230), two VWFC domains (aa271-390), six consecutive EGF-like domains (aa391-631), and three additional C-terminal VWFC domains (aa632 807). Secreted NELL1 forms a 400-420kD noncovalent homotrimer. Over aa17-810, human NELL1 shares 93% aa identity with mouse and rat NELL1. Alternate splicing generates an additional isoform of human NELL1 hat lacks the fifth EGF-like domain.

Product Categories/Family for anti-NELL1 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 46854711
• NCBI GeneID: 4745
• NCBI Official Full Name: Homo sapiens NEL-like 1 (chicken), mRNA
• NCBI Official Synonym Full Names: neural EGFL like 1
• NCBI Official Symbol: NELL1
• NCBI Official Synonym Symbols: NRP1; IDH3GL
• NCBI Protein Information: protein kinase C-binding protein NELL1
• Protein Family: Protein kinase C-binding protein

NCBI Description

This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]