Glucose Regulated Protein 78, Hamster Recombinant Protein
Glucose Regulated Protein 78, Recombinant, Hamster (Grp 78, BiP, Immunoglobulin Heavy Chain Binding Protein)
Applications
Western Blot
Purity
Purified80% pure as determined by SDS-PAGE.
Synonyms
Glucose Regulated Protein 78; Hamster; Recombinant; Hamster (Grp 78; BiP; Immunoglobulin Heavy Chain Binding Protein); Hamster recombinant protein
Host
Recombinant, Hamster (E Coli).
Purity/Purification
Purified
80% pure as determined by SDS-PAGE.
80% pure as determined by SDS-PAGE.
Form/Format
Supplied as a liquid in 37mM Tris-HCl, pH 7.5, 37mM sodium chloride.
Applicable Applications for Glucose Regulated Protein 78, Hamster recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.,
Dilution: Dilute in an SDS containing sample buffer (12) and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 100ng of protein per lane should be sufficient when used in a colorimetric Western Blot.
Dilution: Dilute in an SDS containing sample buffer (12) and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 100ng of protein per lane should be sufficient when used in a colorimetric Western Blot.
Preparation and Storage
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Related Product Information for Glucose Regulated Protein 78, Hamster recombinant protein
Grp78, a 78kD glucose-regulated protein, also known as BiP or immunoglobulin heavy chain binding protein, is a stress-response protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function (2). This protein is essential for the proper glycosylation, folding and assembly of many membrane bound and secreted proteins (3). Grp78 is critical for maintenance of cell homeostasis and the prevention of apoptosis (4). Grp78 protein levels have been shown to be a reliable biomarker of hypoglycemia (5) as well as serving a neuroprotective function in neurons exposed to glutamate and oxidative stress (2). Grp78 levels are reduced in the brains of Alzheimer's Disease patients and decreased expression of Grp78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. The induction of the Grp78 protein has been associated with the development of drug-resistance to anti-tumor drugs (7).
Product Categories/Family for Glucose Regulated Protein 78, Hamster recombinant protein
NCBI and Uniprot Product Information
Uniprot Description
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate ().
Similar Products
Product Notes
The Glucose Regulated Protein 78, Hamster (Catalog #AAA637917) is a Recombinant Protein produced from Recombinant, Hamster (E Coli). and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glucose Regulated Protein 78, Hamster can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot., Dilution: Dilute in an SDS containing sample buffer (12) and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 100ng of protein per lane should be sufficient when used in a colorimetric Western Blot. Researchers should empirically determine the suitability of the Glucose Regulated Protein 78, Hamster for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucose Regulated Protein 78, Hamster, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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