Carbonic Anhydrase III Recombinant Protein | CA3 recombinant protein

Carbonic Anhydrase III, Recombinant, Human (CAIII, CA3, EC 4.2.1.1, Carbonatedehydratase III)

Cat. Num. AAA637459

• Gene Names: CA3; Car3; CAIII
• Purity: Molecular Biology Grade; 95.0% as determined by RP-HPLC, Anion-exchange FPLC, Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Synonyms: Carbonic Anhydrase III; Recombinant; Human (CAIII; CA3; EC 4.2.1.1; Carbonatedehydratase III); CA3 recombinant protein

• Host: Recombinant, Human from E Coli
• Purity/Purification: Molecular Biology Grade; 95.0% as determined by RP-HPLC, Anion-exchange FPLC, Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Form/Format: Supplied as a sterile-filtered, blue solution in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue) and 2.5% glycerol.

• CAS Number: 9001-03-0
• Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis
• Endotoxin Level: 0.1ng/ug (IEU/ug) of CAIII
• Preparation and Storage: Human CAIII although stable at 14 degree C for 1 week, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.

Related Product Information for CA3 recombinant protein

Carbonic anhydrase is widespread in nature, being found in animals, plants, and certain bacteria. In animals it plays an important role in respiration by facilitating transport of CO2 and is involved in the transfer and accumulation of H+ and HCO3-. In chloroplasts of plant cells the role of carbonic anhydrase may be related to photosynthetic fixation of CO2.

Mammalian carbonic anhydrases are of several forms differing in enzymatic properties, amino acid sequences and inhibitor binding. In erythrocytes there is usually present both a high activity and a low activity form. The pH-rate profiles and pH-binding curves indicate that the same group with pKa of approximately seven is involved in both forms. All the isoenzymes have a molecular weight of approximately 30,000 and contain one zinc atom per molecule. Although Deutsch et al. (1972) report six carbonic anhydrases in horse red blood cells, most interest has been focused on high and low activity types. Bovine erythrocytes contain two electrophoretically separable forms designated A and B in order of mobility. Both have a high order of activity similar to the human variant "C". Lindskog et al. (1971) devote a section of their review to polymorphism and nomenclature (pg. 595).
The outstanding characteristic of carbonic anhydrase is its very high turnover number. Zinc may be replaced by cobalt. Shinar and Navon (1974) found that the cobalt-substituted enzyme is dependent upon the state of oxidation of the metal. Appleton and Sarkar (1974) indicate the activity is related to the ionization of a group close to the zinc.
Recombinant Human CAIII, Carbonic anhydrase III having a MW of 33.9kD is one of a group of ten zinc metalloenzymes that catalyse the reversible hydration of CO2 to form HCO3-. CAIII is a cytoplasmic isoenzyme, but is released into the circulation following injury. Recombinant human CAIII is expressed with an amino-terminal hexahistidine tag.

Product Categories/Family for CA3 recombinant protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 179789
• NCBI GeneID: 761
• UniProt Accession #: P07451; O60842; B2R867; B3KUC8
• Molecular Weight: 33.9kD
• NCBI Official Full Name: carbonic anhydrase III
• NCBI Official Synonym Full Names: carbonic anhydrase III, muscle specific
• NCBI Official Symbol: CA3
• NCBI Official Synonym Symbols: Car3; CAIII
• NCBI Protein Information: carbonic anhydrase 3; CA-III; carbonate dehydratase III
• UniProt Protein Name: Carbonic anhydrase 3
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA3
• UniProt Synonym Gene Names: CA-III
• UniProt Entry Name: CAH3_HUMAN

NCBI Description

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]

Uniprot Description

CA3: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding; nickel ion binding

Biological Process: response to ethanol; bicarbonate transport; response to oxidative stress; one-carbon compound metabolic process