CD16 Active Protein | FCGR3A active protein

CD16, Recombinant, Human (Fc gamma RIII alpha, NK cells)

Cat. Num. AAA637279

• Gene Names: FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; FCR-10; FCRIII; FCGRIII; FCRIIIA
• Purity: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1EU/1ug of the protein as determined by the LAL Method.
• Synonyms: CD16; Recombinant; Human (Fc gamma RIII alpha; NK cells); FCGR3A active protein

• Host: NS0 cells
• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1EU/1ug of the protein as determined by the LAL Method.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.

• Activity: Measured by its ability to bind human IgG with an estimated KD < 50nM.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for FCGR3A active protein

Fcy RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes.1, 2 In humans, it is a 50-70kD type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages.1 Fcy RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain (ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils.1, 2 The ECD of Fcy RIIIa shares 63%, 61%, 65%, 59% and 58% aa identity with mouse Fcy RIV, rat Fcy RIIIa, feline CD16, bovine CD16 and porcine Fcy RIIIb paralogs, respectively. The Fcy RIIIa cDNA encodes 254 aa including a 16 aa signal sequence, 191 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 22 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies.3, 4 Fcy RIIIa surface expression requires interaction of an accessory chain, either the common y-chain or CD3c.5, 6 Glycosylation patterns, electrophoretic mobility and binding affinity appear to differ between NK cell and monocyte Fcy RIIIa.7 The ECD of both Fcy RIIIa and b can be proteolytically cleaved and retain binding activity in soluble form.8-11 In monocytes and macrophages, activation and phagocytosis can trigger Fcy RIIIa release.11 Soluble Fcy RIII can be detected in normal plasma and is increased in rheumatoid arthritis and in coronary artery diseases.9, 10

A DNA sequence encoding the extracellular domain of human Fcy RIIIa (Gly 17-Gln 208; Accession # AAH17865) was fused with a six-histidine tag at the C-terminus. The chimeric protein was expressed in a mouse myeloma cell line, NS0.
Calculated molecular mass of approximately 22.6kD. As a result of glycosylation, recombinant human Fcy RIIIa migrates as an approximately 40-50kD protein in SDS-PAGE under reducing conditions.

Product Categories/Family for FCGR3A active protein

Molecular Biology; MB-CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 8176551
• NCBI GeneID: 2214
• UniProt Accession #: P08637; Q53FJ0; Q53FL6; Q5EBR4; Q65ZM6; Q6PIJ0; A2N6W9
• Molecular Weight: 22.6kD
• NCBI Official Full Name: CD16
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• NCBI Official Symbol: FCGR3A
• NCBI Official Synonym Symbols: CD16; FCG3; CD16A; FCGR3; IGFR3; FCR-10; FCRIII; FCGRIII; FCRIIIA
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III-A; FcgammaRIIIA; CD16a antigen; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; igG Fc receptor III-2; Fc gamma receptor III-A; Fc-gamma receptor IIIb (CD16); neutrophil-specific antigen NA; Fc-gamma receptor III-2 (CD 16); immunoglobulin G Fc receptor III; Fc fragment of IgG, low affinity III, receptor for (CD16)
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-A
• Protein Family: CD160 antigen
• UniProt Gene Name: FCGR3A
• UniProt Synonym Gene Names: CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa
• UniProt Entry Name: FCG3A_HUMAN

NCBI Description

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: IgG binding

Biological Process: regulation of immune response; innate immune response; immune response

Disease: Immunodeficiency 20