Rabbit OASL Polyclonal Antibody | anti-OASL antibody

OASL Antibody

Cat. Num. AAA153716

• Gene Names: OASL; OASLd; TRIP14; TRIP-14; p59OASL; p59 OASL; p59-OASL
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: OASL antibody is affinity chromatography purified via peptide column.
• Synonyms: OASL; Polyclonal Antibody; OASL Antibody; OASLd; TRIP14; TRIP-14; p59OASL; p59 OASL; p59-OASL; Thyroid receptor-interacting protein 14; TR-interacting protein 14; 2'-5'-oligoadenylate synthetase-like; anti-OASL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: OASL antibody is human, mouse and rat reactive.
• Purity/Purification: OASL antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 514

• Applicable Applications for anti-OASL antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: OASL antibody can be used for detection of OASL by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunocytochemistry at 2.5 mug/mL. For Immunoflorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: OASL antibody was raised against a 17 amino acid peptide near the amino terminus of human OASL.
• Buffer: OASL Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: OASL antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of OASL in mouse bladder tissue lysate with OASL antibody at 1 μg/ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of OASL1 in HepG2 cells with OASL1 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of OASL1 in HepG2 cells with OASL1 antibody at 20 μg/mL.)

Related Product Information for anti-OASL antibody

OASL (2'-5'-oligoadenylate synthetase-like), also known as p59OASL or TRIP14 (thyroid receptor-interacting protein 14), is a 514 amino acid protein that exists as two alternatively spliced isoforms, designated p56 and p30, and contains two ubiquitin-like domains (1). It is widely expressed in a variety of tissues and interacts with the ligand binding domain of the thyroid receptor (TR) and is able to bind double-stranded RNA and DNA, possibly playing a role in RNA degradation and the overall inhibition of protein synthesis (2-3). Methyl CpG-binding protein 1 (MBD1), which functions as a transcriptional repressor, was identified as a strong p59 OASL interactor (4).

NCBI and Uniprot Product Information

• NCBI GI #: 11321577
• NCBI GeneID: 8638
• NCBI Accession #: NP_003724
• NCBI GenBank Nucleotide #: NM_003733.3
• UniProt Accession #: Q15646; O75686; Q17R95; Q9Y6K6; Q9Y6K7; B2RAZ2; I1YDD2
• Molecular Weight: Predicted: 57 kDa; ; Observed: 65 kDa
• NCBI Official Full Name: 2'-5'-oligoadenylate synthase-like protein isoform a
• NCBI Official Synonym Full Names: 2'-5'-oligoadenylate synthetase-like
• NCBI Official Symbol: OASL
• NCBI Official Synonym Symbols: OASLd; TRIP14; TRIP-14; p59OASL; p59 OASL; p59-OASL
• NCBI Protein Information: 2'-5'-oligoadenylate synthase-like protein; 2'-5'-OAS-RP; 2'-5'-OAS-related protein; TR-interacting protein 14; thyroid receptor-interacting protein 14; 59 kDa 2'-5'-oligoadenylate synthase-like protein; 59 kDa 2'-5'-oligoadenylate synthetase-like protein
• UniProt Protein Name: 2'-5'-oligoadenylate synthase-like protein
• Protein Family: 2'-5'-oligoadenylate synthase-like protein
• UniProt Gene Name: OASL
• UniProt Synonym Gene Names: TRIP14; 2'-5'-OAS-RP; TR-interacting protein 14; TRIP-14; p59OASL
• UniProt Entry Name: OASL_HUMAN

Uniprot Description

OASL: a protein of the 2'-5'-oligoadenylate synthetase family, but does not have 2'-5'-OAS activity. Binds double-stranded RNA and DNA. Interacts with the ligand binding domain of the thyroid receptor (TR). Does not require the presence of thyroid hormone for its interaction. Contains a ubiquitin-like domain that interacts with methyl CpG-binding protein 1.

Protein type: Nucleolus; DNA-binding

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: membrane; cytoplasm; nucleolus; cytosol

Molecular Function: DNA binding; double-stranded RNA binding; thyroid hormone receptor binding; ATP binding; 2'-5'-oligoadenylate synthetase activity

Biological Process: negative regulation of viral genome replication; metabolic process; response to virus; cytokine and chemokine mediated signaling pathway; defense response to virus