Kallikrein 11 Active Protein | KLK11 active protein

Kallikrein 11, Recombinant, Human (KLK11, Hippostasin, Trypsin-like serine protease and PRSS20)

Cat. Num. AAA636984

• Gene Names: KLK11; TLSP; PRSS20
• Purity: Molecular Biology Grade; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Kallikrein 11; Recombinant; Human (KLK11; Hippostasin; Trypsin-like serine protease and PRSS20); KLK11 active protein

• Purity/Purification: Molecular Biology Grade; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a 0.2um filtered solution of 25mM Tris, 10mM CaCl2, 0.15M NaCl, pH 7.5 at the concentration of ~0.7 mg/ml.

• Activity: Measured by its activity to cleave a colormetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405nm and the extinction coefficient of 13,260 M-1cm-1.5
• Specific Activity: Measured with 100uM substrate and 100ng enzyme in 100ul of 50mM Tris, 1M NaCl, 10mM EDTA, pH 8.5, 0.1mM DTNB at RT, is 450pmoles/min/ug.; Activity can be inhibited by AEBSF, dichloroisocoumarin and aprotinin.
• Endotoxin Level: 1EU per 1ug of the protein as determined by the LAL method.
• Preparation and Storage: -70 degree C

Related Product Information for KLK11 active protein

Kallikrein 11 is expressed in brain, skin, ovary and prostate. Increased levels of Kallikrein 11 have been reported in prostate and ovarian carcinoma. It is a serine protease. Kallikrein 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family.6 Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively.1-4. Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain

Product Categories/Family for KLK11 active protein

Molecular Biology; MB-Kallikrein

NCBI and Uniprot Product Information

• NCBI GI #: 262527289
• NCBI GeneID: 11012
• NCBI Accession #: NP_001161077.1
• NCBI GenBank Nucleotide #: NM_001167605.1
• UniProt Accession #: Q9UBX7; O75837; Q0WXX5; Q8IXD7; Q9NS65
• Molecular Weight: The purified, secreted rhKLK11 corresponds to the pro form with an N-terminal sequence of E19TRIIKGFE. By SDS-PAGE under reducing conditions, the apparent molecular mass of the pro form is approximately 40kD.
• NCBI Official Full Name: kallikrein-11 isoform 3
• NCBI Official Synonym Full Names: kallikrein-related peptidase 11
• NCBI Official Symbol: KLK11
• NCBI Official Synonym Symbols: TLSP; PRSS20
• NCBI Protein Information: kallikrein-11; hK11; hippostasin; kallikrein 11; serine protease 20; trypsin-like protease
• UniProt Protein Name: Kallikrein-11
• Protein Family: Kallikrein
• UniProt Gene Name: KLK11
• UniProt Synonym Gene Names: PRSS20; TLSP; hK11
• UniProt Entry Name: KLK11_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in multiple transcript variants encoding distinct isoforms which are differentially expressed.[provided by RefSeq, Nov 2009]

Uniprot Description

KLK11: Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Protease; Secreted, signal peptide; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: Golgi apparatus; extracellular space

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis