Bleomycin Hydrolase Active Protein | BLMH active protein

Bleomycin Hydrolase, Recombinant, Human (BH, BLMH, BMH, BLM Hydrolase)

Cat. Num. AAA636820

• Gene Names: BLMH; BH; BMH
• Purity: Highly Purified; ~90% (SDS-PAGE, reducing conditions)
• Synonyms: Bleomycin Hydrolase; Recombinant; Human (BH; BLMH; BMH; BLM Hydrolase); BLMH active protein

• Purity/Purification: Highly Purified; ~90% (SDS-PAGE, reducing conditions)
• Form/Format: Supplied as a liquid in Tris, sodium chloride, glycerol, DTT.

• Application Notes: SDS-PAGE: 45-50kD, reducing conditions
• Activity: Measured by its ability to hydrolyze Met-AMC.
• Specific Activity: 500pmoles/min/ug.
• Endotoxin Level: 1EU/1ug (LAL)
• Preparation and Storage: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Related Product Information for BLMH active protein

Bleomycin Hydrolase (BLMH) is a cysteine peptidase of the papain superfamily. It is named for its ability to hydrolyze the antitumor agent bleomycin and inactivate it. It has a papain­like catalytic triad (Cys­His­Asp) with optimum activity at neutral pH. In mammals it is expressed ubiquitously in all types of tissues and its expression is up­regulated in many tumors. It is present in the cytoplasm as homohexameric protein of approximately 300kD. In addition to its aminopeptidase activity, it has homocysteine­ thiolactonase activity. The normal physiological function of BLMH is not clear. BLMH inactivates bleomycin, a glycopeptide anticancer agent, by deaminating it. BLMH has been suggested to play a role in the generation of MHC class I­presented peptides. Diminished BLMH activity may contribute to the pathology of Alzheimer's disease (AD) (5­6). It is inhibited by cysteine protease inhibitors such as N­ethylmaleimide, iodoacetamide, para­hydroxymercuribenzoate, and E­64.

Product Categories/Family for BLMH active protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4557367
• NCBI GeneID: 642
• NCBI Accession #: NP_000377.1
• NCBI GenBank Nucleotide #: NM_000386.3
• UniProt Accession #: Q13867; Q53F86; Q9UER9; B2R796
• Molecular Weight: 53 kD
• NCBI Official Full Name: bleomycin hydrolase
• NCBI Official Synonym Full Names: bleomycin hydrolase
• NCBI Official Symbol: BLMH
• NCBI Official Synonym Symbols: BH; BMH
• NCBI Protein Information: bleomycin hydrolase; BLM hydrolase
• UniProt Protein Name: Bleomycin hydrolase
• Protein Family: Bleomycin hydrolase
• UniProt Gene Name: BLMH
• UniProt Synonym Gene Names: BH; BLM hydrolase; BMH
• UniProt Entry Name: BLMH_HUMAN

NCBI Description

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]

Uniprot Description

BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.40; Protease

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; carboxypeptidase activity; cysteine-type endopeptidase activity; aminopeptidase activity; cysteine-type peptidase activity

Biological Process: response to drug; antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; response to toxin; proteolysis

Disease: Alzheimer Disease