Dkk-1, Rat Active Protein
Dkk-1, Recombinant, Rat (Dickkopf 1, Dickkopf 1-like, Dickkopf Homolog 1, Dickkopf-related Protein 1, DKK 1, hDkk 1, SK)
Purity
Highly Purified95% as determined by SDS-PAGE and visualized by silver stain.
Synonyms
Dkk-1; Rat; Recombinant; Rat (Dickkopf 1; Dickkopf 1-like; Dickkopf Homolog 1; Dickkopf-related Protein 1; DKK 1; hDkk 1; SK); Rat active protein
Host
Mouse myeloma cell line, NS0
Purity/Purification
Highly Purified
95% as determined by SDS-PAGE and visualized by silver stain.
95% as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in PBS, 50ug of BSA per 1ug of cytokine. Reconstitute in sterile PBS, 0.1% HSA or BSA to 10ug/ml.
Activity
Able to inhibit Wnt-3A's ability to induce alkaline phosphatase synthesis in the presence of BMP-2 by MC3T3E1, mouse pre-osteoblastic cells. The ND 50 is typically 0.4-1.6ug/ml in the presence of 10ng/ml of rmWnt-3A.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C/-70 degree C. Reconstituted product is stable for 6 months at -20 degree C/-70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Dkk-1, Rat active protein
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. 1, 2 Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues. 1-3 Mature rat Dkk-1 is a 40 kD glycosylated protein that shows 96%, 86% and 82% aa sequence identity with mouse, human and cow Dkk-1, respectively. It also shows 41% and 34% aa identity with rat Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway. 1, 2 This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6. 4 Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2. 4, 5 Dkk-1/LRP6/Krm2 complex internalization has been shown to downregulate Wnt signaling. 4, 5 Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development. 6, 7 Other sites of expression include developing neurons, hair follicles and the retina of the eye. 8, 9 The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively. 8, 10 In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Neuronal expression appears to be required for ischemic neuronal death. 11
Fusion protein composed of Rat Dkk-1 (Ser23-His270) (Accession Number XP_219804, NP_001099820) and 6His expressed in mouse myeloma cell line, NSO.
Fusion protein composed of Rat Dkk-1 (Ser23-His270) (Accession Number XP_219804, NP_001099820) and 6His expressed in mouse myeloma cell line, NSO.
Product Categories/Family for Dkk-1, Rat active protein
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Product Notes
The Dkk-1, Rat (Catalog #AAA636648) is an Active Protein produced from Mouse myeloma cell line, NS0 and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Dkk-1, Rat, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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