Semaphorin 3A Active Protein | SEMA3A active protein

Semaphorin 3A, Recombinant, Mouse (Semaphorin III, Sema III, SEMA3A, SEMAD)

Cat. Num. AAA636480

• Gene Names: SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
• Purity: Purified; 90% (SDS-PAGE)
• Synonyms: Semaphorin 3A; Recombinant; Mouse (Semaphorin III; Sema III; SEMA3A; SEMAD); SEMA3A active protein

• Purity/Purification: Purified; 90% (SDS-PAGE)
• Form/Format: Supplied as a lyophilized powder from PBS, BSA. Carrier free. Reconstitute with 100ug/ml PBS, 0.1% HSA or BSA.

• Biological Activity: Measured by its ability to cause collapse of chick dorsal root ganglia (DRG) neuron growth cones. The ED50 for this effect is typically 20-60ng/ml in the presence of 10ng/ml /beta-NGF.
• Endotoxin Level: 1EU/ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with 100ug/ml PBS, 0.1% HSA or BSA. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for SEMA3A active protein

Semaphorin 3A (Sema3A; previously sem D, sema III or collapsin) is one of six Class 3 secreted semaphorins which share ~40-50% aa identity. Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development. The 772aa mouse Sema3C contains a 20aa signal sequence, an ~500aa N-terminal Sema domain that forms a b-propeller structure similar to that found in integrin molecules, a PSI domain, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain. Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins. The 95kD mature mouse Sema3A shares at least 95% aa identity with human, rat, equine and canine Sema3A, and 90% and 86% aa identity with chick and zebrafish Sema3A, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors. Sema3A signaling is transduced by plexin A1-4, indirectly via neuropilin-1. Sema3A activity is mediated by small GTPases that influence actin rearrangement and integrin activity. It is important in developmental organization of central and peripheral nerves, including those in heart, lung, kidneys, bones, teeth, and visual and olfactory systems. Gradients of Sema3A repel axons, but attract dendrites. Sema3A affect vasculogenesis by inhibiting integrin function and, with Sema3F, promoting apoptosis of endothelial cells. It is thought to suppress cancer- related angiogenesis. In the immune system, Sema3A influences T cell proliferation, migration, response to activation, and interactions with dendritic cells. It negatively regulates platelet activation. Expression of Sema3A in relevant parts of the nervous system may be increased in Alzheimer's disease, multiple sclerosis, ischemia and schizophrenia.

Product Categories/Family for SEMA3A active protein

Molecular Biology; MB-Plexin; Semaphorin

NCBI and Uniprot Product Information

• NCBI GI #: 5174673
• NCBI GeneID: 10371
• NCBI Accession #: NP_006071.1
• NCBI GenBank Nucleotide #: NM_006080.2
• UniProt Accession #: Q14563
• Molecular Weight: 115-120kD
• NCBI Official Full Name: semaphorin-3A
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
• NCBI Official Symbol: SEMA3A
• NCBI Official Synonym Symbols: HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
• NCBI Protein Information: semaphorin-3A; collapsin 1; semaphorin D; semaphorin L; semaphorin III
• UniProt Protein Name: Semaphorin-3A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA3A
• UniProt Synonym Gene Names: SEMAD; Sema III
• UniProt Entry Name: SEM3A_HUMAN

NCBI Description

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p12.1

Cellular Component: membrane; axon; dendrite; extracellular region

Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding

Biological Process: trigeminal nerve structural organization; facial nerve structural organization; axon guidance; apoptosis; regulation of heart rate; olfactory bulb development; dendrite morphogenesis; axonal fasciculation; neuron migration; negative chemotaxis; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; sensory system development

Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia