Rabbit anti-Human, Mouse VSNL1 Polyclonal Antibody | anti-VSNL1 antibody

VSNL1, ID (VSNL1, VISL1, Visinin-like protein 1, Hippocalcin-like protein 3) (MaxLight 650)

Cat. Num. AAA6362859

• Gene Names: VSNL1; HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: VSNL1; Polyclonal Antibody; ID (VSNL1; VISL1; Visinin-like protein 1; Hippocalcin-like protein 3) (MaxLight 650); Hippocalcin-like protein 3; anti-VSNL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VSNL1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-VSNL1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 47-75 in the central region of human VSNL1. Species sequence homology: bovine, chicken, monkey, rat
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of VSNL1 (arrow) in 293 cell lysates (2ug/lane) using MBS6008920 Lane 1: nontransfected Lane 2 transiently transfected with the VSNL1 gene.)

Western Blot (WB)

(Western Blot analysis in mouse heart tissue lysates (35ug/lane) usingMBS6008920. This demonstrates that MBS6008920 detected the VSNL1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6008920 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6008920 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using MBS6008920 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-VSNL1 antibody

Antibodies; Proteins

References

1. Xu, Y., et al. Schizophr. Res. 119 (1-3), 219-227 (2010). 2. Buttgereit, J., et al. Regul. Pept. 161 (1-3), 51-57 (2010). 3. Fu, J., et al. J. Biol. Chem. 284(40):27577-27586(2009). 4. Chen, K.C., et al. J. Pept. Sci. 15(2):89-94(2009). 5. Zhao, C.J., et al. Mol. Cell. Neurosci. 40(2):280-292(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 21361559
• NCBI GeneID: 7447
• NCBI Accession #: NP_003376
• NCBI GenBank Nucleotide #: NM_003385.4
• UniProt Accession #: P62760; P28677; P29103; P42323; Q9UM20; D6W515
• Molecular Weight: 22.1 kDa (191aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: visinin-like protein 1
• NCBI Official Synonym Full Names: visinin like 1
• NCBI Official Symbol: VSNL1
• NCBI Official Synonym Symbols: HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
• NCBI Protein Information: visinin-like protein 1
• UniProt Protein Name: Visinin-like protein 1
• Protein Family: Visinin-like protein
• UniProt Gene Name: VSNL1
• UniProt Synonym Gene Names: VISL1; VILIP; VLP-1; HLP3
• UniProt Entry Name: VISL1_HUMAN

NCBI Description

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

VSNL1: Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner. Belongs to the recoverin family.

Chromosomal Location of Human Ortholog: 2p24.3

Molecular Function: calcium ion binding

Biological Process: calcium-mediated signaling