ST7 Active Protein
ST7, Recombinant, Human (Low-density Lipoprotein Receptor-related Protein 12, LRP-12, Suppressor of Tumorigenicity 7 Protein, LRP12)
Purity
Purified~95% (SDS-PAGE). Endotoxin: <1.0 EU/1ug (LAL).
Synonyms
ST7; Recombinant; Human (Low-density Lipoprotein Receptor-related Protein 12; LRP-12; Suppressor of Tumorigenicity 7 Protein; LRP12); ST7 active protein
Purity/Purification
Purified
~95% (SDS-PAGE). Endotoxin: <1.0 EU/1ug (LAL).
~95% (SDS-PAGE). Endotoxin: <1.0 EU/1ug (LAL).
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute at 200ug/ml in PBS.
Application Notes
SDS-PAGE: 71kD, reducing conditions
Activity
Measured by its binding ability in a functional ELISA. When rhST7 is immobilized at 0.5ug/ml, the concentration of rhLRPAP that produces 50% of the optimal binding response is found to be approximately 0.6-3ug/ml.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for ST7 active protein
ST7 (Suppressor of Tumorigenicity 7), also known as RAY1, TSG7 and FAM4A1, is a type I transmembrane protein belonging to the LDLR superfamily and is designated LRP12. The human ST7 cDNA encodes 859aa including a 32aa signal sequence, a 460aa extracellular domain (ECD) containing two CUB domains and five LDLR class A domains, a 21aa transmembrane domain, and a 346aa cytoplasmic domain containing motifs implicated in endocytosis and signal transduction. Human ST7 shares 95% aa sequence homology with mouse and rat, 96% with canine, and 98% with bovine, equine and porcine ST7 within the ECD. Genomic sequencing indicates the possibility of up to 18 splicing isoforms, but expression of these has not been well-studied. ST7 is widely expressed in normal tissues, especially fibroblasts. Highest mRNA levels were detected in heart and skeletal muscle. ST7 was originally proposed to be a tumor suppressor protein, but it is not consistently downregulated in a variety of cancers, either by mutation or loss of heterozygosity. In certain cancers, expression may even be upregulated. Expression may be associated with downregulated expression of extracellular matrix molecules that are involved in remodeling, such as SPARC, IGFBP5 and several matrix metalloproteinases, and modulation of in vivo tumorigenicity.
Product Categories/Family for ST7 active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
52.7kD
NCBI Official Full Name
ST7 form 2 splice variant b
Protein Family
Uniprot Description
ST7: May act as a tumor suppressor. Belongs to the ST7 family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 7q31.2
Cellular Component: integral to membrane
Similar Products
Product Notes
The ST7 (Catalog #AAA636252) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. SDS-PAGE: 71kD, reducing conditions. Researchers should empirically determine the suitability of the ST7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ST7, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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