Rabbit anti-Human USP6 Polyclonal Antibody | anti-USP6 antibody

USP6, CT (USP6, HRP1, TRE2, Ubiquitin carboxyl-terminal hydrolase 6, Deubiquitinating enzyme 6, Proto-oncogene TRE-2, Ubiquitin thioesterase 6, Ubiquitin-specific-processing protease 6) (MaxLight 650)

Cat. Num. AAA6362012

• Gene Names: USP6; HRP1; TRE2; TRE17; Tre-2; TRESMCR; USP6-short
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: USP6; Polyclonal Antibody; CT (USP6; HRP1; TRE2; Ubiquitin carboxyl-terminal hydrolase 6; Deubiquitinating enzyme 6; Proto-oncogene TRE-2; Ubiquitin thioesterase 6; Ubiquitin-specific-processing protease 6) (MaxLight 650); Ubiquitin-specific-processing protease 6; anti-USP6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP6.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-USP6 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1280-1310 in the C-terminal region of human USP6.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H460 cells using MBS6000961 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-USP6 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Product Categories/Family for anti-USP6 antibody

Antibodies; Ubiquitin

References

1. Paulding, C.A., et al., Proc. Natl. Acad. Sci. U.S.A. 100(5):2507-2511 (2003). 2. Papa, F.R., et al., Nature 366(6453):313-319 (1993). 3. Nakamura, T., et al., Oncogene 7(4):733-741 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 747165331
• NCBI GeneID: 9098
• NCBI Accession #: NP_001291213.1
• NCBI GenBank Nucleotide #: NM_001304284.1
• UniProt Accession #: P35125; Q15634; Q86WP6; Q8IWT4
• Molecular Weight: 89,577 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 6
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 6
• NCBI Official Symbol: USP6
• NCBI Official Synonym Symbols: HRP1; TRE2; TRE17; Tre-2; TRESMCR; USP6-short
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 6
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 6
• Protein Family: USP6 N-terminal-like protein
• UniProt Gene Name: USP6
• UniProt Synonym Gene Names: HRP1; TRE2

Uniprot Description

USP6: Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6- dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation. A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Oncoprotein; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: cytoplasm; lysosome; plasma membrane; recycling endosome

Molecular Function: calmodulin binding; cysteine-type endopeptidase activity; nucleic acid binding; protein binding; ubiquitin-specific protease activity

Biological Process: protein deubiquitination