Rabbit anti-Human USP43 Polyclonal Antibody | anti-USP43 antibody

USP43, CT (USP43, Ubiquitin carboxyl-terminal hydrolase 43, Deubiquitinating enzyme 43, Ubiquitin thioesterase 43, Ubiquitin-specific-processing protease 43) (Biotin)

Cat. Num. AAA6361962

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: USP43; Polyclonal Antibody; CT (USP43; Ubiquitin carboxyl-terminal hydrolase 43; Deubiquitinating enzyme 43; Ubiquitin thioesterase 43; Ubiquitin-specific-processing protease 43) (Biotin); Ubiquitin-specific-processing protease 43; anti-USP43 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-USP43 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: USP43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 899-928 amino acids from the C-terminal region of human USP43.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(USP43 Antibody (C-term) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the USP43 antibody detected the USP43 protein (arrow).)

Immunohistochemistry (IHC)

(USP43 Antibody (C-term) (AP14283b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USP43 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-USP43 antibody

USP43 may recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins (By similarity).

Product Categories/Family for anti-USP43 antibody

Antibodies; Ubiquitin

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Quesada, V., et al. Biochem. Biophys. Res. Commun. 314(1):54-62(2004). Puente, X.S., et al. Nat. Rev. Genet. 4(7):544-558(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 148612811
• NCBI GeneID: 124739
• NCBI Accession #: NP_694942.3
• NCBI GenBank Nucleotide #: NM_153210.4
• UniProt Accession #: Q70EL4; Q8N2C5; Q96DQ6; A6NDT9; B7ZLT9; B7ZVX5
• Molecular Weight: 122,294 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 43 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 43
• NCBI Official Symbol: USP43
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 43; ubiquitin thioesterase 43; deubiquitinating enzyme 43; ubiquitin thiolesterase 43; ubiquitin specific protease 43; ubiquitin-specific-processing protease 43
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 43
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP43
• UniProt Entry Name: UBP43_HUMAN

Uniprot Description

USP43: May recognize and hydrolyze the peptide bond at the C- terminal Gly of ubiquitin. Involved in the processing of poly- ubiquitin precursors as well as that of ubiquitinated proteins. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Ubiquitin-specific protease; Protease

Chromosomal Location of Human Ortholog: 17p13.1

Molecular Function: cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination