Eck, N-terminal His tag Active Protein | EPHA2 active protein

Eck, Recombinant, Human, N-terminal His tag (EphA2, Mpk-5, Native Epithelial Cell Kinase, Eph receptor tyrosine kinase A2)

Cat. Num. AAA636065

• Gene Names: EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
• Reactivity: Human
• Purity: 80% by SDS-PAGE
• Synonyms: Eck; N-terminal His tag; Recombinant; Human; N-terminal His tag (EphA2; Mpk-5; Native Epithelial Cell Kinase; Eph receptor tyrosine kinase A2); EPHA2 active protein

• Reactivity: Human
• Purity/Purification: 80% by SDS-PAGE
• Form/Format: 25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.

• Application Notes: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Specific Activity: 170 U/mg. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30 degree C.
• Assay Buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 30ug/ml Poly (Glu:Tyr)4:1 substrate, and 0.1ug /ml recombinant EPHA2.
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Related Product Information for EPHA2 active protein

Human EPHA2 (GenBank Accession No. NM_004431), (a.a. 596-end) with N-terminal His tag.

MW 47kD, expressed in an Baculovirus infected Sf9 cell expression system.

Product Categories/Family for EPHA2 active protein

Molecular Biology; MB-Receptors; Tyrosine Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 32967311
• NCBI GeneID: 1969
• NCBI Accession #: NP_004422.2
• NCBI GenBank Nucleotide #: NM_004431.3
• UniProt Accession #: P29317; Q8N3Z2
• Molecular Weight: 47kD
• NCBI Official Full Name: ephrin type-A receptor 2
• NCBI Official Synonym Full Names: EPH receptor A2
• NCBI Official Symbol: EPHA2
• NCBI Official Synonym Symbols: ECK; CTPA; ARCC2; CTPP1; CTRCT6
• NCBI Protein Information: ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase
• UniProt Protein Name: Ephrin type-A receptor 2
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA2
• UniProt Synonym Gene Names: ECK
• UniProt Entry Name: EPHA2_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]

Uniprot Description

EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice.

Protein type: Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Kinase, protein; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: focal adhesion; cell surface; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; ephrin receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: neural tube development; axial mesoderm formation; axon guidance; cell migration; peptidyl-tyrosine phosphorylation; negative regulation of protein kinase B signaling cascade; viral reproduction; multicellular organismal development; notochord formation; mammary gland epithelial cell proliferation; osteoclast differentiation; bone remodeling; regulation of blood vessel endothelial cell migration; regulation of angiogenesis; osteoblast differentiation; keratinocyte differentiation; regulation of cell adhesion mediated by integrin; DNA damage response, signal transduction resulting in induction of apoptosis; notochord cell development; protein kinase B signaling cascade; ephrin receptor signaling pathway; angiogenesis; vasculogenesis; cell adhesion; skeletal development

Disease: Cataract 6, Multiple Types