Rabbit anti-Human TTL Polyclonal Antibody | anti-TTL antibody

TTL, CT (TTL, Tubulin-tyrosine ligase) (Azide free) (HRP)

Cat. Num. AAA6360028

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TTL; Polyclonal Antibody; CT (TTL; Tubulin-tyrosine ligase) (Azide free) (HRP); Tubulin--tyrosine ligase; anti-TTL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TTL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-385 amino acids from the C-terminal region of human TTL.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TTL Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the TTL antibody detected the TTL protein (arrow).)

Related Product Information for anti-TTL antibody

TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).

Product Categories/Family for anti-TTL antibody

Antibodies; Tubulin

References

Dick, D.M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010): Erck, C., et al. Cytogenet. Genome Res. 101(1):47-53(2003). Mialhe, A., et al. Cancer Res. 61(13):5024-5027(2001). Ersfeld, K., et al. J. Cell Biol. 120(3):725-732(1993).

NCBI and Uniprot Product Information

• NCBI GI #: 24308516
• NCBI GeneID: 150465
• NCBI Accession #: NP_714923.1
• NCBI GenBank Nucleotide #: NM_153712.4
• UniProt Accession #: Q8NG68; Q585T3; Q7Z302; Q8N426
• Molecular Weight: 43,212 Da
• NCBI Official Full Name: tubulin--tyrosine ligase
• NCBI Official Synonym Full Names: tubulin tyrosine ligase
• NCBI Official Symbol: TTL
• NCBI Protein Information: tubulin--tyrosine ligase; 2410003M22Rik; tubulin-tyrosine ligase
• UniProt Protein Name: Tubulin--tyrosine ligase
• Protein Family: TITAN-like protein
• UniProt Gene Name: TTL
• UniProt Synonym Gene Names: TTL
• UniProt Entry Name: TTL_HUMAN

NCBI Description

TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM, Mar 2008]

Uniprot Description

TTL: Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin. Belongs to the tubulin--tyrosine ligase family.

Protein type: EC 6.3.2.25; Cell development/differentiation; Ligase

Chromosomal Location of Human Ortholog: 2q13

Molecular Function: tubulin-tyrosine ligase activity; ATP binding

Biological Process: protein modification process; regulation of axon extension; microtubule cytoskeleton organization and biogenesis