Galectin-3 Binding Protein Active Protein | LGALS3 active protein

Galectin-3 Binding Protein, Recombinant, Mouse (MAC-2BP)

Cat. Num. AAA635971

• Gene Names: LGALS3; Gal-3; CBP 35; Galectin-3
• Purity: >90% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Galectin-3 Binding Protein; Recombinant; Mouse (MAC-2BP); LGALS3 active protein

• Purity/Purification: >90% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder from 20mM MOPS, 100mM sodium chloride, 10mM Imidazole, 5% trehalose. Reconstitute with 167ul sterile PBS

• Biological Activity: Measured by its binding ability in a functional ELISA. When rmMAC-2BP is coated at 1ug/ml (100ul/well), rhGalectin-3 binds with an apparent KD <5nM.
• Endotoxin Level: <0.1EU/ug (LAL)
• Source: Recombinant protein corresponding to Thr19-Val577 with a C-terminal 6-His tag from mouse Galectin-3 Binding Protein, expressed in NS0 cells. (NP_035280)
• SDS-PAGE: 80-90kD, reducing conditions.
• Preparation and Storage: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for LGALS3 active protein

Galectin-3 binding protein (Galectin-3BP), called CyCAP (cyclophilin C-associated protein) in the mouse, is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) super family. Mouse Galectin-3BP is generally considered the paralog of human Galectin-3BP, which is also called MAC-2 binding protein (MAC-2BP), or 90kD tumor associated antigen (TAA90K or 90K). However, mouse Galectin-3BP binds cyclophilin C but not Galectin-3, while the opposite is true of human Galectin-3BP. The 577 amino acid, 77-95kD mouse Galectin-3BP contains an 18aa signal sequence and four definitive domains. Domain 1 is a group A scavenger receptor domain, domain 2 is a BTB/POZ domain that mediates dimerization, and domain 3 is an IVR domain that is also found following the POZ domain in Drosophila kelch protein. Little is known about domain 4. Mature mouse Galectin-3BP shares 88%, 69%, 67% and 65% aa identity with rat, human, dog and cow Galectin-3BP, respectively. The Galectin-3BP dimer interface residues are generally conserved between mouse and human, so linear dimers that occur in humans are also likely in the mouse. Human Galectin-3BP also forms ring-shaped decamers and dodecamers. Mouse Galectin-3BP is widely expressed and modifies innate immune reactions. It is stimulated in macrophages by adhesion, TNF-a or IFN-g. Mice deficient in Galectin-3BP secrete increased amounts of IL-12, TNF-a and IFN-g and have lower survival in response to endotoxin. Galectin-3BP is induced in rat skin fibroblasts, keratinocytes, macrophages and lymphocytes during wound healing, and wounded Galectin-3BP-deficient mice are slow to heal. Rat Galectin-3BP is upregulated after cerebral ischemia. Ligands for human Galectin-3BP include some other galectins, collagens and integrins, but remain to be tested for mouse Galectin-3BP binding.

Product Categories/Family for LGALS3 active protein

Molecular Biology; MB-Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 308081799
• NCBI GeneID: 404021
• NCBI Accession #: NP_001183972.1
• NCBI GenBank Nucleotide #: NM_001197043.1
• UniProt Accession #: P38486
• Molecular Weight: 63.3kD
• NCBI Official Full Name: galectin-3
• NCBI Official Symbol: LGALS3
• NCBI Official Synonym Symbols: Gal-3; CBP 35; Galectin-3
• NCBI Protein Information: galectin-3; lectin L-29; 35 kDa lectin; mac-2 antigen; igE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35; beta-galactosides-binding lectin; lectin, galactoside-binding, soluble, 3 (galectin 3)
• UniProt Protein Name: Galectin-3
• UniProt Gene Name: LGALS3
• UniProt Synonym Gene Names: Gal-3; CBP 35
• UniProt Entry Name: LEG3_CANFA

Uniprot Description

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Motility/polarity/chemotaxis; Cell surface; Extracellular matrix

Cellular Component: spliceosome; cytoplasm; extracellular region

Molecular Function: IgE binding; carbohydrate binding

Biological Process: RNA splicing; innate immune response; mRNA processing; cell differentiation