DnaK, aa385-546, Substrate Binding Domain, E. coli Recombinant Protein
DnaK, aa385-546, Substrate Binding Domain, Recombinant, E. coli
Purity
Highly Purified95% by SDS PAGE; Purified by using conventional chromatography techniques.
Synonyms
DnaK; aa385-546; Substrate Binding Domain; E. coli; Recombinant; E. coli recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
95% by SDS PAGE; Purified by using conventional chromatography techniques.
95% by SDS PAGE; Purified by using conventional chromatography techniques.
Form/Format
Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol and 1mM EDTA.
Sequence
MDVKDVLLLD/ VTPLSLGIET/ MGGVMTTLIA/ KNTTIPTKHS/ QVFSTAEDNQ/ SAVTIHVLQG/ ERKRAADNKS/ LGQFNLDGIN/ PAPRGMPQIE/ VTFDIDADGI/ LHVSAKDKNS/ GKEQKITIKA/ SSGLNEDEIQ/ KMVRDAEANA/ EADRKFEELV/ QTRNQGDHLL/ HST
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for DnaK, aa385-546, Substrate Binding Domain, E. coli recombinant protein
DNAK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli. Additional amino acid (Met) is attached at N- terminus.
Product Categories/Family for DnaK, aa385-546, Substrate Binding Domain, E. coli recombinant protein
NCBI and Uniprot Product Information
Uniprot Description
Acts as a chaperone.
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Product Notes
The DnaK, aa385-546, Substrate Binding Domain, E. coli (Catalog #AAA635780) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MDVKDVLLLD / VTPLSLGIET / MGGVMTTLIA / KNTTIPTKHS / QVFSTAEDNQ / SAVTIHVLQG / ERKRAADNKS / LGQFNLDGIN / PAPRGMPQIE / VTFDIDADGI / LHVSAKDKNS / GKEQKITIKA / SSGLNEDEIQ / KMVRDAEANA / EADRKFEELV / QTRNQGDHLL / HST. It is sometimes possible for the material contained within the vial of "DnaK, aa385-546, Substrate Binding Domain, E. coli, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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