WNT-11 active protein
Wnt-11, Recombinant, Human (Protein Wnt-11, Wnt11)
Purity
Purified~95% by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1.0 EU/ug (LAL).
Synonyms
Wnt-11; Recombinant; Human (Protein Wnt-11; Wnt11); WNT-11 active protein
Host
CHO cells
Purity/Purification
Purified
~95% by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1.0 EU/ug (LAL).
~95% by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1.0 EU/ug (LAL).
Form/Format
Supplied as a powder from PBS, 0.1mM EDTA, 1% CHAPS, pH 7.0, 50ug BSA. Reconstitute with 100ul sterile PBS, 0.1% HSA or BSA.
Activity
Measured in a cell proliferation assay using IEC-18 rat small intestinal epithelial cell line. The ED50 for this effect is typically 0.6-2.4ug/ml.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for WNT-11 active protein
Wnt-11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation. Mature human Wnt-11 shares 98% aa sequence identity with mouse, rat and bovine Wnt-11, and 99%, 96% and 88% aa identity with canine, equine and chick Wnt-11, respectively. Mouse Wnt-11 is expressed in early development during gastrulation and in the perichondrium, lung mesenchyme and ureteric buds, but not in neuroepithelium. It is required for cardiogenesis, organization of early muscle fibers, and branching morphogenesis of ureters. It is also expressed in several human cancers. In vitro, Wnt-11 has been shown to induce cardiomyocyte differentiation from unfractionated mouse bone marrow mononuclear cells, and to promote hematopoietic differentiation from human embryoid body stem cells by engaging Frizzled-7 in the presence of E-cadherin. In general, there are three signaling pathways associated with Wnt-receptor interaction. The canonical pathway results in b-Catenin accumulation and TCF/LEF-1- mediated gene transcription. Non-canonical pathways include the Wnt/Ca2+ pathway and the planar cell polarity (PCP) pathway. Wnt-11 often, but not exclusively, signals by a non-canonical pathway and may repress canonical signaling by other Wnts. However, when complexed with Wnt-5a, Wnt-11 can also enhance canonical signaling.
Recombinant protein corresponding to aa25-354 of human wNT-11 expressed in CHO cells (NP_004617).
Recombinant protein corresponding to aa25-354 of human wNT-11 expressed in CHO cells (NP_004617).
Product Categories/Family for WNT-11 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36.6kD
NCBI Official Full Name
WNT-11 protein
NCBI Official Symbol
WNT-11
NCBI Protein Information
WNT-11 protein
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Product Notes
The WNT-11 (Catalog #AAA635774) is an Active Protein produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Wnt-11, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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