OGG1, aa1-345, Recombinant Protein | OGG1 recombinant protein

OGG1, Recombinant, Human, aa1-345, (8-Oxoguanine DNA Glycosylase, HMMH, HOGG1, MUTM, OGH1, AP lyase)

Cat. Num. AAA635773

• Gene Names: OGG1; HMMH; MUTM; OGH1; HOGG1
• Purity: Purified; Chromatographically purified (90%), SDS-PAGE.
• Synonyms: OGG1; aa1-345; ; Recombinant; Human; (8-Oxoguanine DNA Glycosylase; HMMH; HOGG1; MUTM; OGH1; AP lyase); OGG1 recombinant protein

• Host: E Coli
• Purity/Purification: Purified; Chromatographically purified (90%), SDS-PAGE.
• Form/Format: Supplied as a sterile-filtered colorless solution. 20mM Tris-HCl buffer pH8, 0.1M NaCl, 40% glycerol.
• Sequence: MGSSHHHHHH SSGLVPRGSH TGSMPARALL PRRMGHRTLA STPALWASIP CPRSELRLDL VLPSGQSFRW REQSPAHWSG VLADQVWTLT QTEEQLHCTV YRGDKSQASR PTPDELEAVR KYFQLDVTLA QLYHHWGSVD SHFQEVAQKF QGVRLLRQDP IECLFSFICS SNNNIARITG MVERLCQAFG PRLIQLDDVT YHGFPSLQAL AGPEVEAHLR KLGLGYRARY VSASARAILE EQGGLAWLQQ LRESSYEEAH KALCILPGVG TKVADCICLM ALDKPQAVPV DVHMWHIAQRDYSWHPTTSQ AKGPSPQTNK ELGNFFRSLW GPYAGWAQAV LFSADLRQCR HAQEPPAKRRKGSKGPEG.

• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Related Product Information for OGG1 recombinant protein

OGG1 is a DNA glycosylase enzyme which takes part in base excision repair. OGG1 protein is the main enzyme accountable for the excision of 7,8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which arises as a result of exposure to reactive oxygen species (ROS). OGG1 shows beta lyase activity that nicks DNA 3'' to the lesion.

OGG1 Recombinant Human produced in E. coli is a single, non-glycosylated polypeptide chain containing 368 amino acids (aa1-345) and having a molecular mass of 41.2kD. The OGG1 is fused to 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Categories/Family for OGG1 recombinant protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4587158
• NCBI GeneID: 4968
• UniProt Accession #: O15527; O00390; O00670; O00705; O14876; O95488; P78554; Q9BW42; Q9UIK0; Q9UIK1; Q9UIK2; A8K1E3
• Molecular Weight: 41.2D
• NCBI Official Full Name: OGG1 protein type 1b
• NCBI Official Synonym Full Names: 8-oxoguanine DNA glycosylase
• NCBI Official Symbol: OGG1
• NCBI Official Synonym Symbols: HMMH; MUTM; OGH1; HOGG1
• NCBI Protein Information: N-glycosylase/DNA lyase; AP lyase; OGG1 type 1f; 8-hydroxyguanine DNA glycosylase; DNA-apurinic or apyrimidinic site lyase
• UniProt Protein Name: N-glycosylase/DNA lyase
• Protein Family: N-glycosylase/DNA lyase
• UniProt Gene Name: OGG1
• UniProt Synonym Gene Names: MMH; MUTM; OGH1; AP lyase
• UniProt Entry Name: OGG1_HUMAN

NCBI Description

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]

Uniprot Description

OGG1: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3' to the lesion. Defects in OGG1 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Belongs to the type-1 OGG1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Lyase; Deoxyribonuclease; EC 4.2.99.18

Chromosomal Location of Human Ortholog: 3p26.2

Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; nuclear speck

Molecular Function: protein binding; microtubule binding; endonuclease activity; DNA N-glycosylase activity; oxidized purine base lesion DNA N-glycosylase activity; damaged DNA binding; 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity

Biological Process: response to drug; depurination; DNA repair; DNA catabolic process, endonucleolytic; response to estradiol stimulus; response to radiation; response to ethanol; base-excision repair, AP site formation; regulation of transcription, DNA-dependent; nucleotide-excision repair; base-excision repair; response to folic acid; regulation of protein import into nucleus, translocation; response to oxidative stress; acute inflammatory response; negative regulation of apoptosis; aging

Disease: Renal Cell Carcinoma, Nonpapillary