Rabbit anti-Human OGG1 Polyclonal Antibody | anti-OGG1 antibody

OGG1 (N-glycosylase/DNA Lyase, 8-oxoguanine DNA Glycosylase, DNA-(Apurinic or Apyrimidinic Site) Lyase, AP Lyase, MMH, MUTM, OGH1) (Biotin)

Cat. Num. AAA6387944

• Gene Names: OGG1; HMMH; MUTM; OGH1; HOGG1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OGG1; Polyclonal Antibody; OGG1 (N-glycosylase/DNA Lyase; 8-oxoguanine DNA Glycosylase; DNA-(Apurinic or Apyrimidinic Site) Lyase; AP Lyase; MMH; MUTM; OGH1) (Biotin); anti-OGG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OGG1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-OGG1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human OGG1, aa1-384 (NP_058212.1).
• Immunogen Sequence: MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVSVPRCPP
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(OGG1 rabbit polyclonal antibody. Western Blot analysis of OGG1 expression in Jurkat.)

Western Blot (WB)

(Western Blot analysis of OGG1 expression in transfected 293T cell line by OGG1 polyclonal antibody. Lane 1: OGG1 transfected lysate (36.4kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-OGG1 antibody

OGG1, also known as 8-oxoguanine glycosylase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7,8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS). It has a beta lyase activity that nicks DNA 3' to the lesion.

Product Categories/Family for anti-OGG1 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 8670530
• NCBI GeneID: 4968
• NCBI Accession #: NP_058212.1
• NCBI GenBank Nucleotide #: NM_016819.2
• UniProt Accession #: O15527; O00390; O00670; O00705; O14876; O95488; P78554; Q9BW42; Q9UIK0; Q9UIK1; Q9UIK2; A8K1E3
• Molecular Weight: 36,085 Da
• NCBI Official Full Name: N-glycosylase/DNA lyase isoform 1b
• NCBI Official Synonym Full Names: 8-oxoguanine DNA glycosylase
• NCBI Official Symbol: OGG1
• NCBI Official Synonym Symbols: HMMH; MUTM; OGH1; HOGG1
• NCBI Protein Information: N-glycosylase/DNA lyase; AP lyase; OGG1 type 1f; 8-hydroxyguanine DNA glycosylase; DNA-apurinic or apyrimidinic site lyase
• UniProt Protein Name: N-glycosylase/DNA lyase
• Protein Family: N-glycosylase/DNA lyase
• UniProt Gene Name: OGG1
• UniProt Synonym Gene Names: MMH; MUTM; OGH1; AP lyase
• UniProt Entry Name: OGG1_HUMAN

NCBI Description

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]

Uniprot Description

OGG1: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3' to the lesion. Defects in OGG1 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Belongs to the type-1 OGG1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Deoxyribonuclease; EC 4.2.99.18; DNA repair, damage

Chromosomal Location of Human Ortholog: 3p26.2

Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; nuclear speck

Molecular Function: protein binding; endonuclease activity; microtubule binding; oxidized purine base lesion DNA N-glycosylase activity; DNA N-glycosylase activity; damaged DNA binding; 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity

Biological Process: response to drug; depurination; DNA repair; DNA catabolic process, endonucleolytic; response to estradiol stimulus; response to radiation; response to ethanol; base-excision repair, AP site formation; regulation of transcription, DNA-dependent; base-excision repair; nucleotide-excision repair; response to folic acid; regulation of protein import into nucleus, translocation; response to oxidative stress; acute inflammatory response; negative regulation of apoptosis; aging

Disease: Renal Cell Carcinoma, Nonpapillary