Rabbit anti-Human TNPO1 Polyclonal Antibody | anti-TNPO1 antibody

TNPO1, NT (TNPO1, KPNB2, MIP1, TRN, Transportin-1, Importin beta-2, Karyopherin beta-2, M9 region interaction protein) (APC)

Cat. Num. AAA6357275

• Gene Names: TNPO1; MIP; TRN; IPO2; MIP1; KPNB2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TNPO1; Polyclonal Antibody; NT (TNPO1; KPNB2; MIP1; TRN; Transportin-1; Importin beta-2; Karyopherin beta-2; M9 region interaction protein) (APC); M9 region interaction protein; anti-TNPO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TNPO1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TNPO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45~75 amino acids from the N-terminal region of human TNPO1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TNPO1 Antibody (N-term) in 293 cell line lysates (35ug/lane). TNPO1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with TNPO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TNPO1 Antibody (N-term) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TNPO1 antibody

TNPO1 comprises the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins.

Product Categories/Family for anti-TNPO1 antibody

Antibodies; Transport Proteins

References

Fineberg, K., et al., Biochemistry 42(9):2625-2633 (2003). Nelson, L.M., et al., Virology 306(1):162-169 (2003). Le Roux, L.G., et al., J. Virol. 77(4):2330-2337 (2003). Limon, A., et al., J. Virol. 76(21):10598-10607 (2002). Dvorin, J.D., et al., J. Virol. 76(23):12087-12096 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 133925811
• NCBI GeneID: 3842
• NCBI Accession #: NP_002261.3
• NCBI GenBank Nucleotide #: NM_002270.3
• UniProt Accession #: Q92973; Q92957; Q92975; B4DVC6
• Molecular Weight: 96,901 Da
• NCBI Official Full Name: transportin-1 isoform 1
• NCBI Official Synonym Full Names: transportin 1
• NCBI Official Symbol: TNPO1
• NCBI Official Synonym Symbols: MIP; TRN; IPO2; MIP1; KPNB2
• NCBI Protein Information: transportin-1; importin 2; importin beta 2; importin beta-2; karyopherin beta-2; M9 region interaction protein; karyopherin (importin) beta 2
• UniProt Protein Name: Transportin-1
• UniProt Gene Name: TNPO1
• UniProt Synonym Gene Names: KPNB2; MIP1; TRN; MIP
• UniProt Entry Name: TNPO1_HUMAN

NCBI Description

This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

KPNB2: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in nuclear import of M9- containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Belongs to the importin beta family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nuclear import; Karyopherin

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding; nuclear localization sequence binding; Ran GTPase binding

Biological Process: viral reproduction; protein import into nucleus, translocation; organelle organization and biogenesis; gene expression