Rabbit anti-Human TLE4 Polyclonal Antibody | anti-TLE4 antibody

TLE4, NT (TLE4, KIAA1261, Transducin-like enhancer protein 4) (Biotin)

Cat. Num. AAA6355802

• Gene Names: TLE4; ESG; BCE1; ESG4; GRG4; BCE-1; E(spI)
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TLE4; Polyclonal Antibody; NT (TLE4; KIAA1261; Transducin-like enhancer protein 4) (Biotin); Transducin-like enhancer protein 4; anti-TLE4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TLE4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TLE4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-196 amino acids from the N-terminal region of human TLE4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TLE4 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the TLE4 antibody detected the TLE4 protein (arrow).)

Related Product Information for anti-TLE4 antibody

TLE4 is a transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).

Product Categories/Family for anti-TLE4 antibody

Antibodies; KIAA; orf

References

Liu, Y., et al. PLoS Genet. 5 (11), E1000707 (2009): Hancock, D.B., et al. PLoS Genet. 5 (8), E1000623 (2009): Stolk, L., et al. Nat. Genet. (2009). In press: Dayyani, F., et al. Blood 111(8):4338-4347(2008). Heimbucher, T., et al. Mol. Cell. Biol. 27(1):340-351(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 34915994
• NCBI GeneID: 7091
• NCBI Accession #: NP_008936.2
• NCBI GenBank Nucleotide #: NM_007005.3
• UniProt Accession #: Q04727; Q3ZCS1; Q5T1Y2; Q6PCB3; Q9BZ07; Q9BZ08; Q9BZ09; Q9NSL3; Q9ULF9
• Molecular Weight: 83,755 Da
• NCBI Official Full Name: transducin-like enhancer protein 4
• NCBI Official Synonym Full Names: transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)
• NCBI Official Symbol: TLE4
• NCBI Official Synonym Symbols: ESG; BCE1; ESG4; GRG4; BCE-1; E(spI)
• NCBI Protein Information: transducin-like enhancer protein 4; Protein BCE-1; B lymphocyte gene 1; enhancer of split groucho 4; transducin-like enhancer of split 4, homolog of Drosophila E(sp1)
• UniProt Protein Name: Transducin-like enhancer protein 4
• Protein Family: Transducin-like enhancer protein
• UniProt Gene Name: TLE4
• UniProt Synonym Gene Names: KIAA1261
• UniProt Entry Name: TLE4_HUMAN

Uniprot Description

TLE4: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Homooligomer and heterooligomer with other family members. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2. Interacts with ZNF703; TLE4 may mediate ZNF703 transcriptional repression. In all tissues examined, mostly in brain, and muscle. Belongs to the WD repeat Groucho/TLE family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9q21.31

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin binding; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; Notch signaling pathway; Wnt receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter