Rabbit anti-Human TFAP4 Polyclonal Antibody | anti-TFAP4 antibody

TFAP4, CT (TFAP4, BHLHC41, Transcription factor AP-4, Activating enhancer-binding protein 4, Class C basic helix-loop-helix protein 41) (AP)

Cat. Num. AAA6354975

• Gene Names: TFAP4; AP-4; bHLHc41
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TFAP4; Polyclonal Antibody; CT (TFAP4; BHLHC41; Transcription factor AP-4; Activating enhancer-binding protein 4; Class C basic helix-loop-helix protein 41) (AP); Class C basic helix-loop-helix protein 41; anti-TFAP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TFAP4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TFAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-342 amino acids from the C-terminal region of human TFAP4.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TFAP4 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the TFAP4 antibody detected the TFAP4 protein (arrow).)

Immunohistochemistry (IHC)

(TFAP4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TFAP4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TFAP4 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TFAP4 antibody

Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization.

Product Categories/Family for anti-TFAP4 antibody

Antibodies; Transcription Factors

References

Ku, W.C., et al. Mol. Cell Proteomics 8(9):2034-2050(2009). Jung, P., et al. Proc. Natl. Acad. Sci. U.S.A. 105(39):15046-15051(2008). Pons, J., et al. Biochemistry 47(1):14-29(2008). Mermod, N., et al. Nature 332(6164):557-561(1988).

NCBI and Uniprot Product Information

• NCBI GI #: 4507447
• NCBI GeneID: 7023
• NCBI Accession #: NP_003214
• NCBI GenBank Nucleotide #: NM_003223
• UniProt Accession #: Q01664
• Molecular Weight: 39kDa
• NCBI Official Full Name: transcription factor AP-4
• NCBI Official Synonym Full Names: transcription factor AP-4
• NCBI Official Symbol: TFAP4
• NCBI Official Synonym Symbols: AP-4; bHLHc41
• NCBI Protein Information: transcription factor AP-4
• UniProt Protein Name: Transcription factor AP-4
• Protein Family: Transcription factor
• UniProt Gene Name: TFAP4
• UniProt Synonym Gene Names: BHLHC41; bHLHc41
• UniProt Entry Name: TFAP4_HUMAN

NCBI Description

Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM, Jul 2009]