Testing Data
(No inflamatory response after DNA delivery using in vivo Transfection Reagent.|50ug of plasmid DNA was delivered into female BALB/c mice via intravenous injection using in vivo Transfection Reagent. The concentration of proinflamatory cytokine TNFα was determined by ELISA in blood serum samples taken at the indicated time points. LPS – lipopolysaccharides.)
Transfection Reagent
Transfection Reagent (in vivo)
Synonyms
Transfection Reagent; Transfection Reagent (in vivo); Transfection Reagent reagent
Quality Control
Transfection efficiency was tested on BALB/c female mice (7-8 weeks old) using 50ug Firefly luciferase coding plasmid and 6ul of in vivo Transfection reagents per mouse. The delivery of the transgene was evaluated by measurement of Firefly luciferase activity in total protein extracted from the lungs of transfected animals. 100% of tested mice expressed the Firefly luciferase.
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(No inflamatory response after DNA delivery using in vivo Transfection Reagent.|50ug of plasmid DNA was delivered into female BALB/c mice via intravenous injection using in vivo Transfection Reagent. The concentration of proinflamatory cytokine TNFα was determined by ELISA in blood serum samples taken at the indicated time points. LPS – lipopolysaccharides.)
Testing Data
(No inflamatory response after DNA delivery using in vivo Transfection Reagent.|50ug of plasmid DNA was delivered into female BALB/c mice via intravenous injection using in vivo Transfection Reagent. The concentration of proinflamatory cytokine TNFα was determined by ELISA in blood serum samples taken at the indicated time points. LPS – lipopolysaccharides.)
Testing Data
(Comparison of in vivo Transfection Reagent with other in vivo transfection reagents.|50ug of plasmid DNA coding for firefly luciferase was complexed with in vivo transfection reagents and delivered into female BALB/c mice via intravenous injection. Transfections were performed according to manufacturer's recommendations. Firefly luciferase expression was determined in lungs 24 h after plasmid DNA delivery.)
Testing Data
(Comparison of in vivo Transfection Reagent with other in vivo transfection reagents.|50ug of plasmid DNA coding for firefly luciferase was complexed with in vivo transfection reagents and delivered into female BALB/c mice via intravenous injection. Transfections were performed according to manufacturer's recommendations. Firefly luciferase expression was determined in lungs 24 h after plasmid DNA delivery.)
Related Product Information for Transfection Reagent reagent
Transfection Reagent (in vivo) is highly effective for DNA plasmid delivery in vivo, while not inducing an inflammatory response. TurboFect in vivo has been assessed for plasmid DNA delivery in both mice and rats and is suitable for DNA administration via various routes, including intravenous, intraperitoneal, and intratumoral injection. The amount of DNA and suggested injection volume will depend on the experimental animal and the route of administration as well as the targeted tissue or organ and the expression vector.
Transfection Reagent (in vivo) is a sterile solution of a cationic polymer in water. The polymer forms compact, stable, positively charged complexes with DNA. These complexes protect DNA from degradation and facilitate gene delivery in vivo. Reagent causes no detectable inflamatory response and is suitable for DNA administration via various routes, including intravenous, intraperitoneal, intratumoral injection, etc.
Transfection Reagent (in vivo) for 20 transfections using 50ug DNA.
Transfection Reagent (in vivo) is a sterile solution of a cationic polymer in water. The polymer forms compact, stable, positively charged complexes with DNA. These complexes protect DNA from degradation and facilitate gene delivery in vivo. Reagent causes no detectable inflamatory response and is suitable for DNA administration via various routes, including intravenous, intraperitoneal, intratumoral injection, etc.
Transfection Reagent (in vivo) for 20 transfections using 50ug DNA.
Product Categories/Family for Transfection Reagent reagent
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Product Notes
The Transfection Reagent (Catalog #AAA635395) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Transfection Reagent, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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